Cannon J G, Angel J B, Abad L W, O'Grady J, Lundgren N, Fagioli L, Komaroff A L
Department of Medicine, New England Medical Center, Boston, Massachusetts, USA.
J Clin Immunol. 1998 Jul;18(4):291-8. doi: 10.1023/a:1027389907780.
This investigation tested the hypotheses that women diagnosed with chronic fatigue syndrome (CFS) would exhibit significantly greater systemic indices of exercise-induced leukocyte mobilization and inflammation (neutrophilia, lactoferrin release, complement activation) than controls matched for age, weight, and habitual activity and that responses in the luteal phase of the menstrual cycle would be greater than in the follicular phase. Subjects stepped up and down on a platform adjusted to the height of the patella for 15 min, paced by metronome. Blood samples were collected under basal conditions (the day before exercise) and following exercise for determination of circulating neutrophils and plasma concentrations of lactoferrin, C3a des arg, and creatine kinase. Complete, 24-hr urine collections were made for determination of cortisol excretion. For all subjects, circulating neutrophil counts increased 33% (P < 0.0001) and lactoferrin increased 27% (P = 0.0006) after exercise, whereas plasma C3a des arg and creatine kinase did not increase. No indication of an exaggerated or excessive response was observed in the CFS patients compared to the controls. In healthy women, circulating neutrophil numbers exhibited previously described relationships with physiological variables: basal neutrophil counts correlated with plasma progesterone concentrations (R = 0.726, P = 0.003) and the exercise-induced neutrophilia correlated with both urinary cortisol (R = 0.660, P = 0.007) and plasma creatine kinase (R = 0.523, P = 0.038) concentrations. These relationships were not observed in the CFS patients (R = 0.240, P = 0.370; R = 0.042, P = 0.892; and R = 0.293, P = 0.270; respectively). These results suggest that normal endocrine influences on the circulating neutrophil pool may be disrupted in patients with CFS.
被诊断为慢性疲劳综合征（CFS）的女性，与年龄、体重和日常活动量相匹配的对照组相比，会表现出运动诱导的白细胞动员和炎症（中性粒细胞增多、乳铁蛋白释放、补体激活）的全身指标显著更高，且月经周期黄体期的反应会大于卵泡期。受试者在一个根据髌骨高度调整的平台上随着节拍器节奏上下踏步15分钟。在基础条件下（运动前一天）和运动后采集血样，以测定循环中性粒细胞以及乳铁蛋白、C3a去精氨酸和肌酸激酶的血浆浓度。收集24小时的完整尿液以测定皮质醇排泄量。对所有受试者而言，运动后循环中性粒细胞计数增加了33%（P<0.0001），乳铁蛋白增加了27%（P = 0.0006），而血浆C3a去精氨酸和肌酸激酶未增加。与对照组相比，未观察到CFS患者有过度或过激反应的迹象。在健康女性中，循环中性粒细胞数量呈现出与生理变量先前描述的关系：基础中性粒细胞计数与血浆孕酮浓度相关（R = 0.726，P = 0.003），运动诱导的中性粒细胞增多与尿皮质醇（R = 0.660，P = 0.007）和血浆肌酸激酶（R = 0.523，P = 0.038）浓度均相关。在CFS患者中未观察到这些关系（分别为R = 0.240，P = 0.370；R = 0.042，P = 0.892；以及R = 0.293，P = 0.270）。这些结果表明，CFS患者中正常的内分泌对循环中性粒细胞池的影响可能受到破坏。
