Erlandsen E J, Randers E, Kristensen J H
Department of Clinical Biochemistry, Viborg County Hospital, Denmark.
Clin Chem Lab Med. 1998 Jun;36(6):393-7. doi: 10.1515/CCLM.1998.067.
The aim of this study was to establish reference intervals for cerum cystatin C and serum creatinine in adults. Blood samples were collected from 270 healthy blood donors (135 men and 135 women between 20 and 65 years old with 15 men and 15 women in each five-year-interval). Serum cystatin C was analyzed using an automated particle-enhanced immunoassay (DAKO Cystatin C PET kit) on the Cobas Mira S analyzer. Serum creatinine was analyzed using the Vitros Creatinine Slide, an enzymatic method on the Vitros 950 chemistry analyzer. The calculated reference intervals for serum cystatin C were 0.62-1.15 mg/l in women (median 0.84 mg/l, range 0.56-1.29 mg/l) and 0.51-1.25 mg/l in men (median 0.87 mg/l, range 0.42-1.39 mg/l). The Mann-Whithey U-test revealed no gender-related difference for cystatin C (p = 0.48). A common reference interval in women and men was calculated to be 0.54-1.21 mg/l (median 0.85 mg/l, range 0.42-1.39 mg/l). The non-parametric reference interval for serum creatinine was 57-95 mumol/l in women (median 72 mumol/l, range 44-105 mumol/l) and 69-111 mumol/l in men (median 89 mumol/l, range 58-123 mumol/l).
本研究的目的是建立成人血清胱抑素C和血清肌酐的参考区间。从270名健康献血者（135名男性和135名女性，年龄在20至65岁之间，每5年年龄区间有15名男性和15名女性）采集血样。使用Cobas Mira S分析仪上的自动颗粒增强免疫测定法（DAKO胱抑素C PET试剂盒）分析血清胱抑素C。使用Vitros肌酐载玻片在Vitros 950化学分析仪上采用酶法分析血清肌酐。计算得出女性血清胱抑素C的参考区间为0.62 - 1.15mg/l（中位数0.84mg/l，范围0.56 - 1.29mg/l），男性为0.51 - 1.25mg/l（中位数0.87mg/l，范围0.42 - 1.39mg/l）。曼 - 惠特尼U检验显示胱抑素C无性别相关差异（p = 0.48）。计算得出男性和女性的共同参考区间为0.54 - 1.21mg/l（中位数0.85mg/l，范围0.42 - 1.39mg/l）。血清肌酐的非参数参考区间女性为57 - 95μmol/l（中位数72μmol/l，范围44 - 105μmol/l），男性为69 - 111μmol/l（中位数89μmol/l，范围58 - 123μmol/l）。
