早期tRNA加工的核仁定位
Nucleolar localization of early tRNA processing.
作者信息
Bertrand E, Houser-Scott F, Kendall A, Singer R H, Engelke D R
机构信息
Institut de Genetique Moleculaire de Montpellier-Centre National de la Recherche Scientifique (CNRS), 34033 Montpellier Cedex 01, France.
出版信息
Genes Dev. 1998 Aug 15;12(16):2463-8. doi: 10.1101/gad.12.16.2463.
Abstract
There is little information as to the location of early tRNA biosynthesis. Using fluorescent in situ hybridization in the budding yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are shown to reside primarily in the nucleoli. We also probed the RNA subunit of RNase P. The majority of the signal from RNase P probes was nucleolar, with less intense signals in the nucleoplasm. These results demonstrate that a major portion of the tRNA processing pathway is compartmentalized in nucleoli with rRNA synthesis and ribosomal assembly. The spatial juxtaposition suggests the possibility of direct coordination between tRNA and ribosome biosynthesis.
摘要
关于早期tRNA生物合成的位置，目前所知甚少。利用荧光原位杂交技术在芽殖酵母酿酒酵母中进行研究，发现核内前体tRNA主要位于核仁中。我们还检测了核糖核酸酶P的RNA亚基。核糖核酸酶P探针的大部分信号位于核仁，核质中的信号较弱。这些结果表明，tRNA加工途径的主要部分与rRNA合成和核糖体组装一起被分隔在核仁中。这种空间上的并列表明tRNA和核糖体生物合成之间可能存在直接协调。
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1
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EMBO J. 1998 Jul 1;17(13):3747-57. doi: 10.1093/emboj/17.13.3747.
2
Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP.核酶RNase P全酶复合物的纯化与特性分析揭示了其与RNase MRP在亚基上存在广泛重叠。
Genes Dev. 1998 Jun 1;12(11):1678-90. doi: 10.1101/gad.12.11.1678.
3
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4
Genetic and physical maps of Saccharomyces cerevisiae.酿酒酵母的遗传图谱和物理图谱。
Nature. 1997 May 29;387(6632 Suppl):67-73.
5
Nuclear domains of the RNA subunit of RNase P.核糖核酸酶P的RNA亚基的核结构域。
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