Roberson K M, Yancey D R, Padilla-Nash H, Edwards D W, Nash W, Jacobs S, Padilla G M, Larchian W A, Robertson C N
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
In Vitro Cell Dev Biol Anim. 1998 Jul-Aug;34(7):537-44. doi: 10.1007/s11626-998-0113-y.
Molecular studies of bladder carcinomas have aided in determining causative genetic events and the prognosis of cancers endowed with certain abnormalities. In vitro bladder cancer characterization of key cytogenetic alterations is useful for study of molecular changes that may promote oncogenic events. In our laboratory, a novel human bladder cancer cell line, BK10, has been established in vitro and passaged for more than 20 mo. This new bladder cancer cell line (BK10) was derived from bladder tissue containing grade III-IV/IV transitional cell carcinoma. Bladder cancer tissue was obtained at the time of radical cystoprostatectomy extirpation. Cell cultures derived from this surgical sample exhibited an epithelial morphology and expressed epithelial cytokeratins. Immunostains of BK10 were negative for prostate specific antigen (PSA), fibronectin, smooth muscle actin alpha, and desmin. Karyotypic analysis revealed an aneuploid chromosomal content <4n> with many numerical and structural abnormalities previously linked to bladder oncogenesis. Translocations occurred in chromosomes 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 13, 14, 15, 16, 17, 19, 20, 21, 22, X and Y. G-banding analysis revealed rearrangements involving chromosomes 9q and 17p, and the location of the ab11 oncogene and the p53 gene, respectively. The availability of this bladder cancer cell line will provide a useful tool for the further study of bladder carcinoma oncogenesis and gene therapy.
膀胱癌的分子研究有助于确定致癌基因事件以及具有某些异常特征癌症的预后。对关键细胞遗传学改变进行体外膀胱癌特征分析，有助于研究可能促进致癌事件的分子变化。在我们实验室，一种新型人膀胱癌细胞系BK10已在体外建立并传代超过20个月。这种新的膀胱癌细胞系（BK10）源自含有III-IV/IV级移行细胞癌的膀胱组织。在根治性膀胱前列腺切除术切除时获取膀胱癌组织。从该手术样本衍生的细胞培养物呈现上皮形态并表达上皮细胞角蛋白。BK10的免疫染色对前列腺特异性抗原（PSA）、纤连蛋白、平滑肌肌动蛋白α和结蛋白呈阴性。核型分析显示非整倍体染色体含量<4n>，有许多先前与膀胱肿瘤发生相关的数量和结构异常。染色体1、2、3、4、5、6、7、8、9、11、13、14、15、16、17、19、20、21、22、X和Y发生易位。G带分析分别显示涉及染色体9q和17p的重排，以及abl1癌基因和p53基因的位置。这种膀胱癌细胞系的可用性将为进一步研究膀胱癌的肿瘤发生和基因治疗提供有用的工具。
