BACTEC系统与盲目传代培养法在检测菌血症方面的比较。
Comparison of the BACTEC system with blind subculture for the detection of bacteremia.
作者信息
Caslow M, Ellner P D, Kiehn T E
出版信息
Appl Microbiol. 1974 Sep;28(3):435-8. doi: 10.1128/am.28.3.435-438.1974.
Abstract
One thousand blood specimens were cultured in BACTEC vials containing modified Columbia broth in aerobic, anaerobic, and hypertonic formulations. Radiometric readings and subcultures were performed on aerobic and hypertonic vials at 24 h and 7 days, and on anaerobic vials at 48 h and 7 days. Significant numbers of false-positive BACTEC readings were obtained. Although all positive cultures were eventually detected by the BACTEC, approximately 20% of blood specimens yielding positive subcultures at 24 h did not give positive BACTEC readings until 48 h.
摘要
1000份血液标本在含有改良哥伦比亚肉汤的BACTEC瓶中进行需氧、厌氧和高渗配方培养。对需氧和高渗瓶在24小时和7天时进行放射性读数和传代培养，对厌氧瓶在48小时和7天时进行放射性读数和传代培养。获得了大量假阳性的BACTEC读数。尽管所有阳性培养物最终都被BACTEC检测到，但约20%在24小时产生阳性传代培养的血液标本直到48小时才出现阳性BACTEC读数。
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