拟南芥抗病基因RPS5富含亮氨酸重复结构域内的一个突变部分抑制了多个细菌和霜霉病抗性基因。
A mutation within the leucine-rich repeat domain of the Arabidopsis disease resistance gene RPS5 partially suppresses multiple bacterial and downy mildew resistance genes.
作者信息
Warren R F, Henk A, Mowery P, Holub E, Innes R W
机构信息
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
出版信息
Plant Cell. 1998 Sep;10(9):1439-52. doi: 10.1105/tpc.10.9.1439.
Abstract
Recognition of pathogens by plants is mediated by several distinct families of functionally variable but structurally related disease resistance (R) genes. The largest family is defined by the presence of a putative nucleotide binding domain and 12 to 21 leucine-rich repeats (LRRs). The function of these LRRs has not been defined, but they are speculated to bind pathogen-derived ligands. We have isolated a mutation in the Arabidopsis RPS5 gene that indicates that the LRR region may interact with other plant proteins. The rps5-1 mutation causes a glutamate-to-lysine substitution in the third LRR and partially compromises the function of several R genes that confer bacterial and downy mildew resistance. The third LRR is relatively well conserved, and we speculate that it may interact with a signal transduction component shared by multiple R gene pathways.
摘要
植物对病原体的识别是由几个不同的基因家族介导的，这些基因家族功能各异但结构相关，被称为抗病（R）基因。最大的家族的特征是存在一个假定的核苷酸结合结构域和12到21个富含亮氨酸的重复序列（LRR）。这些LRR的功能尚未明确，但推测它们能结合病原体衍生的配体。我们在拟南芥RPS5基因中分离出一个突变，这表明LRR区域可能与其他植物蛋白相互作用。rps5-1突变导致第三个LRR中的谷氨酸被赖氨酸取代，部分损害了几个赋予细菌和霜霉病抗性的R基因的功能。第三个LRR相对保守，我们推测它可能与多个R基因途径共享的信号转导成分相互作用。
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Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region.亚麻锈病抗性基因M的失活与富含亮氨酸重复序列编码区域内一个重复单元的缺失有关。
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