Wojewódzka M, Kruszewski M, Iwaneñko T, Collins A R, Szumiel I
Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland.
Mutat Res. 1998 Aug 7;416(1-2):21-35. doi: 10.1016/s1383-5718(98)00073-4.
We examined a group of people professionally at risk of exposure to low doses of ionizing radiation (altogether 49 individuals). Age, use of therapeutic drugs, work-related exposure to hazardous agents, previous exposures to diagnostic X-rays, such as patient and nuclear medical examination, were registered. For each individual, the occupational radiation burden received over the past period of 5 years was taken from the official personal records based on film dosimetry controlled every month. A matched group of controls was chosen among the administrative employees (40 individuals). The mean age of the studied population at the time of blood sampling was 49 years (range 24-69). The individuals were divided into groups according to risk of exposure and sex. The alkaline comet assay was used to measure DNA breaks and alkali-labile sites. We compared the mean tail moments, tail length and percentage of DNA in the tail. There was a significant difference between the control and hazard groups in DNA damage. Higher DNA damage was also found for men than for women in the control group. There was no relation of DNA damage to age either in control or hazard group. Additionally, analysis of distributions of tail moment values pointed to a considerable individual diversity even in the control group. Therefore, further investigations were necessary into the suitability of the comet assay as a biological dosimetry method; the results obtained so far warrant such investigations.
我们对一组职业上有接触低剂量电离辐射风险的人员（共49人）进行了检查。记录了他们的年龄、治疗药物使用情况、工作中接触有害剂的情况、既往接受诊断性X射线检查（如患者和核医学检查）的情况。对于每个人，过去5年的职业辐射负担是从基于每月控制的胶片剂量测定法的官方个人记录中获取的。在行政员工中选取了一组匹配的对照组（40人）。采血时研究人群的平均年龄为49岁（范围24 - 69岁）。根据接触风险和性别将个体分为不同组。采用碱性彗星试验来测量DNA断裂和碱不稳定位点。我们比较了平均尾矩、尾长和尾中DNA百分比。对照组和危险组在DNA损伤方面存在显著差异。在对照组中，男性的DNA损伤也高于女性。在对照组和危险组中，DNA损伤与年龄均无关联。此外，尾矩值分布分析表明，即使在对照组中也存在相当大的个体差异。因此，有必要进一步研究彗星试验作为生物剂量测定方法的适用性；目前获得的结果证明了此类研究的必要性。
