Lioi M B, Scarfì M R, Santoro A, Barbieri R, Zeni O, Di Berardino D, Ursini M V
Dipartimento di Scienze delle Produzioni Animali, Università della Basilicata, Potenza, Italy.
Mutat Res. 1998 Jul 17;403(1-2):13-20. doi: 10.1016/s0027-5107(98)00010-4.
The genotoxic activity of the pesticides gliphosate, vinclozolin and DPX-E9636 was studied in in vitro cultures of bovine lymphocytes, using chromosome aberration (CA) and sister chromatid exchange (SCE) frequencies as genetic end-points and a variation of glucose 6-phosphate dehydrogenase (G6PD) enzyme activity as a marker of changes in the normal cell redox state. Results indicated a statistically significant increase of structural aberrations, sister chromatid exchanges and G6PD activity, suggesting that the pesticides tested induce either oxidative stress or a mutagenic effect in this species. The evaluation of both mitotic index and cell viability, after pesticide exposure, demonstrates a high cytotoxic effect which is always associated with the observed genotoxic effect.
以染色体畸变（CA）和姐妹染色单体交换（SCE）频率作为遗传终点，并以葡萄糖6 - 磷酸脱氢酶（G6PD）酶活性的变化作为正常细胞氧化还原状态变化的标志物，在牛淋巴细胞的体外培养中研究了农药草甘膦、乙烯菌核利和DPX - E9636的遗传毒性活性。结果表明，结构畸变、姐妹染色单体交换和G6PD活性在统计学上有显著增加，表明所测试的农药在该物种中诱导了氧化应激或诱变效应。农药暴露后对有丝分裂指数和细胞活力的评估表明，其具有高细胞毒性作用，且该作用总是与观察到的遗传毒性作用相关。
