噬菌体T7内通过曲率应力稳定的六角形堆积DNA。
Hexagonally packed DNA within bacteriophage T7 stabilized by curvature stress.
作者信息
Odijk T
机构信息
Faculty of Chemical Engineering and Materials Science, Delft University of Technology, The Netherlands.
出版信息
Biophys J. 1998 Sep;75(3):1223-7. doi: 10.1016/S0006-3495(98)74041-X.
Abstract
A continuum computation is proposed for the bending stress stabilizing DNA that is hexagonally packed within bacteriophage T7. Because the inner radius of the DNA spool is rather small, the stress of the curved DNA genome is strong enough to balance its electrostatic self-repulsion so as to form a stable hexagonal phase. The theory is in accord with the microscopically determined structure of bacteriophage T7 filled with DNA within the experimental margin of error.
摘要
提出了一种连续介质计算方法，用于计算稳定噬菌体T7内六边形堆积的DNA的弯曲应力。由于DNA卷轴的内半径相当小，弯曲的DNA基因组的应力足够强，足以平衡其静电自排斥力，从而形成稳定的六边形相。该理论在实验误差范围内与微观测定的充满DNA的噬菌体T7的结构一致。
相似文献
1
Hexagonally packed DNA within bacteriophage T7 stabilized by curvature stress.噬菌体T7内通过曲率应力稳定的六角形堆积DNA。
Biophys J. 1998 Sep;75(3):1223-7. doi: 10.1016/S0006-3495(98)74041-X.
2
Conformation and interactions of the packaged double-stranded DNA genome of bacteriophage T7.噬菌体T7包装的双链DNA基因组的构象与相互作用。
Biospectroscopy. 1998;4(5 Suppl):S47-56. doi: 10.1002/(SICI)1520-6343(1998)4:5+<S47::AID-BSPY6>3.0.CO;2-7.
3
4
Electrostatic map of T7 DNA: comparative analysis of functional and electrostatic properties of T7 RNA polymerase-specific promoters.T7 DNA的静电图谱：T7 RNA聚合酶特异性启动子的功能和静电特性的比较分析
J Biomol Struct Dyn. 2014;32(8):1184-92. doi: 10.1080/07391102.2013.819298. Epub 2013 Jul 30.
5
Single-event analysis of the packaging of bacteriophage T7 DNA concatemers in vitro.噬菌体T7 DNA多联体体外包装的单事件分析。
Biophys J. 1999 Sep;77(3):1627-37. doi: 10.1016/S0006-3495(99)77011-6.
6
[Kink dynamics in inhomogeneous DNA].[非均匀DNA中的扭结动力学]
Biofizika. 2008 Jan-Feb;53(1):36-41.
7
Defects in concatemer processing of bacteriophage T7 DNA deleted in the M-hairpin region.在M发夹区域缺失的噬菌体T7 DNA的多联体加工缺陷。
Virology. 1997 Sep 15;236(1):37-46. doi: 10.1006/viro.1997.8715.
8
Conformational changes leading to T7 DNA delivery upon interaction with the bacterial receptor.与细菌受体相互作用时导致T7 DNA传递的构象变化。
J Biol Chem. 2015 Apr 17;290(16):10038-44. doi: 10.1074/jbc.M114.614222. Epub 2015 Feb 19.
9
DNA buckling in bacteriophage cavities as a mechanism to aid virus assembly.噬菌体腔内的DNA弯曲作为辅助病毒组装的一种机制。
J Struct Biol. 2015 Mar;189(3):251-8. doi: 10.1016/j.jsb.2015.01.002. Epub 2015 Jan 19.
10
Can Changes in Temperature or Ionic Conditions Modify the DNA Organization in the Full Bacteriophage Capsid?温度或离子条件的变化会改变完整噬菌体衣壳中的DNA组织吗？
J Phys Chem B. 2016 Jul 7;120(26):5975-86. doi: 10.1021/acs.jpcb.6b01783. Epub 2016 May 6.
引用本文的文献
1
Theoretical Studies on Assembly, Physical Stability, and Dynamics of Viruses.病毒组装、物理稳定性及动力学的理论研究
Subcell Biochem. 2024;105:693-741. doi: 10.1007/978-3-031-65187-8_19.
2
'SAXS-osmometer' method provides measurement of DNA pressure in viral capsids and delivers an empirical equation of state.'SAXS-osmometer' 方法提供了病毒衣壳中 DNA 压力的测量，并提供了一个经验状态方程。
Nucleic Acids Res. 2023 Nov 27;51(21):11415-11427. doi: 10.1093/nar/gkad852.
3
Ion-induced changes in DNA gels.离子诱导的 DNA 凝胶变化。
Soft Matter. 2023 Jul 19;19(28):5405-5415. doi: 10.1039/d3sm00666b.
4
Nonequilibrium dynamics and ultraslow relaxation of confined DNA during viral packaging.病毒包装过程中受限 DNA 的非平衡动力学和超慢弛豫。
Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8345-50. doi: 10.1073/pnas.1405109111. Epub 2014 May 27.
5
Polymorphism of DNA conformation inside the bacteriophage capsid.噬菌体衣壳内DNA构象的多态性。
J Biol Phys. 2013 Mar;39(2):201-13. doi: 10.1007/s10867-013-9315-y. Epub 2013 Apr 12.
6
Entropy, energy, and bending of DNA in viral capsids.病毒衣壳中 DNA 的熵、能量和弯曲。
Biophys J. 2013 May 21;104(10):L15-7. doi: 10.1016/j.bpj.2013.04.006.
7
Communication: Origin of the contributions to DNA structure in phages.交流：噬菌体中对 DNA 结构贡献的起源。
J Chem Phys. 2013 Feb 21;138(7):071103. doi: 10.1063/1.4791708.
8
Single-molecule studies of viral DNA packaging.单分子水平上的病毒 DNA 包装研究。
Adv Exp Med Biol. 2012;726:549-84. doi: 10.1007/978-1-4614-0980-9_24.
9
Ion-dependent dynamics of DNA ejections for bacteriophage lambda.噬菌体 λ DNA 喷射的离子依赖性动力学。
Biophys J. 2010 Aug 9;99(4):1101-9. doi: 10.1016/j.bpj.2010.06.024.
10
Visualization of bacteriophage T3 capsids with DNA incompletely packaged in vivo.体内DNA未完全包装的T3噬菌体衣壳的可视化。
J Mol Biol. 2008 Dec 31;384(5):1384-99. doi: 10.1016/j.jmb.2008.10.012. Epub 2008 Oct 14.
本文引用的文献
1
X-ray diffraction studies of bacterial viruses.细菌病毒的X射线衍射研究。
Nature. 1961 Sep 23;191:1242-5. doi: 10.1038/1911242a0.
2
The conformation of packaged bacteriophage T7 DNA: informative images of negatively stained T7.包装的噬菌体T7 DNA的构象：经负染色的T7的信息性图像。
J Struct Biol. 1997 Oct;120(1):32-43. doi: 10.1006/jsbi.1997.3895.
3
Encapsidated conformation of bacteriophage T7 DNA.噬菌体T7 DNA的衣壳化构象
Cell. 1997 Oct 17;91(2):271-80. doi: 10.1016/s0092-8674(00)80409-2.
4
The conformation of DNA packaged in bacteriophage G.包装在噬菌体G中的DNA的构象
Biophys J. 1997 Feb;72(2 Pt 1):958-63. doi: 10.1016/s0006-3495(97)78730-7.
5
Structure of viral connectors and their function in bacteriophage assembly and DNA packaging.病毒连接体的结构及其在噬菌体组装和DNA包装中的功能。
Q Rev Biophys. 1994 May;27(2):107-155. doi: 10.1017/s0033583500004510.
6
Polymer- and salt-induced toroids of hexagonal DNA.聚合物和盐诱导的六方DNA环面
Biophys J. 1995 Jan;68(1):54-61. doi: 10.1016/S0006-3495(95)80158-X.
7
Assembly of bacteriophage T7. Dimensions of the bacteriophage and its capsids.噬菌体T7的组装。噬菌体及其衣壳的尺寸。
Biophys J. 1981 Dec;36(3):743-57. doi: 10.1016/S0006-3495(81)84763-7.
8
Packaging of DNA into bacteriophage heads: a model.DNA包装进噬菌体头部：一个模型
J Mol Biol. 1983 Dec 25;171(4):577-80. doi: 10.1016/0022-2836(83)90045-1.
9
Cryo-electron microscopy of viruses.病毒的冷冻电子显微镜技术
Nature. 1984;308(5954):32-6. doi: 10.1038/308032a0.
10
Tests of spool models for DNA packaging in phage lambda.λ噬菌体中DNA包装的线轴模型测试。
J Mol Biol. 1983 Dec 25;171(4):419-37. doi: 10.1016/0022-2836(83)90038-4.
Zotero 插件