Ordal G W, Villani D P, Nicholas R A, Hamel F G
J Biol Chem. 1978 Jul 25;253(14):4916-9.
Values of KI for nine proline analogs as inhibitors of proline chemotaxis and of proline transport were determined. Two of them inhibited transport at substantially lower concentrations than chemotaxis; two at substantially higher concentrations. Moreover, mutants, believed to be in the component that binds proline, were isolated that showed a shift of KM for transport to higher concentrations, one as much as 40-fold. However, chemotaxis was virtually unaffected. Therefore, unlike galactose chemotaxis and transport in Escherichia coli, which share the galactose-binding protein, proline chemotaxis and transport in Bacillus subtilis are independent.
测定了九种脯氨酸类似物作为脯氨酸趋化作用抑制剂和脯氨酸转运抑制剂的抑制常数（KI）值。其中两种在比趋化作用低得多的浓度下抑制转运；两种在高得多的浓度下抑制转运。此外，分离出了被认为是脯氨酸结合成分发生突变的菌株，这些菌株显示转运的米氏常数（KM）向更高浓度偏移，其中一个高达40倍。然而，趋化作用几乎不受影响。因此，与大肠杆菌中共享半乳糖结合蛋白的半乳糖趋化作用和转运不同，枯草芽孢杆菌中的脯氨酸趋化作用和转运是独立的。
