Plebani M, De Toni S, Sanzari M C, Bernardi D, Stockreiter E
Department of Laboratory Medicine, University-Hospital of Padua, Italy.
Am J Clin Pathol. 1998 Sep;110(3):334-40. doi: 10.1093/ajcp/110.3.334.
We evaluated performance of the TEST 1 (SIRE Analytical Systems, Udine, Italy), a fully automated analyzer for the measurement of the erythrocyte sedimentation rate (ESR). Intra-assay reproducibility was satisfactory for a wide range of ESR values, whereas there was a significant decrease in ESR data when the samples were stored at 4 degrees C for up to 24 hours. We compared TEST 1 with the Westergren ESR method approved by the International Council for Standardization in Haematology (ICSH) and with the Diesse Ves-Matic analyzer Linear regression analysis comparing the TEST 1 and the ICSH reference method yielded satisfactory correlations for K3 EDTA- and sodium citrate-anticoagulated samples. Bland-Altman analysis showed no evidence of a systematic bias between the TEST 1 and the reference method. A close correlation was found between the TEST 1 system and the Diesse Ves-Matic analyzer despite a significant positive systematic bias. Reference values for men and women were analyzed according to nonparametric statistics. The TEST 1 was easy to use, had a satisfactory operative practicability required minimal maintenance, and reduced contact with potential biohazards. This system enables the determination of ESR with any common standard-sized tube; the use of samples anticoagulated with K3 EDTA can widely reduce the workload in clinical laboratories.
我们评估了TEST 1（意大利乌迪内的SIRE分析系统）的性能，这是一款用于测量红细胞沉降率（ESR）的全自动分析仪。在广泛的ESR值范围内，批内重复性令人满意，然而，当样本在4℃下储存长达24小时时，ESR数据显著下降。我们将TEST 1与国际血液学标准化委员会（ICSH）认可的魏氏ESR方法以及Diesse Ves-Matic分析仪进行了比较。对TEST 1和ICSH参考方法进行线性回归分析，结果显示对于用K3乙二胺四乙酸（EDTA）和柠檬酸钠抗凝的样本，二者具有令人满意的相关性。布兰德-奥特曼分析表明，TEST 1与参考方法之间没有系统偏差的迹象。尽管存在显著的正系统偏差，但TEST 1系统与Diesse Ves-Matic分析仪之间仍发现密切相关性。根据非参数统计分析了男性和女性的参考值。TEST 1易于使用，具有令人满意的操作实用性，所需维护极少，并减少了与潜在生物危害的接触。该系统能够使用任何常见标准尺寸的试管测定ESR；使用K3 EDTA抗凝的样本可广泛减少临床实验室的工作量。
