Arikan Serap, Akalin Nalan
Biochemistry Department, Baskent University, Ankara, Turkey.
Ann Saudi Med. 2007 Sep-Oct;27(5):362-5. doi: 10.5144/0256-4947.2007.362.
The erythrocyte sedimentation rate (ESR) remains the most widely used laboratory test for monitoring infections, inflammatory diseases and some types of cancer. Several test methods have been developed recently, and as a result, the safety and reliability of ESR testing procedures have improved. The purpose of this study was the comparison of two methods, the traditional manual Westergren method (reference method of the International Committee on Standardization in Hematology) and a new semiautomated technique, the Micro Test 1 for determining the ESR.
Blood samples were collected after a night's fasting from 200 hospitalized and ambulatory patients. Undiluted blood samples anticoagulated with K3 EDTA that had Micro Test 1 values ranging from 2-82 mm/h were used for comparison with the Westergren method.
Linear regression analysis comparing the Micro Test 1 and the reference method yielded satisfactory correlations and regression for samples (r=0.910; P=0.0001; y=4.91+0.86 x; Sy/x=6.85). A Bland-Altman analysis showed no evidence of systematic bias between the Micro Test 1 and the reference method.
The Micro Test 1 system was easy to use, had a satisfactory operative practicability, required minimal maintenance, and reduced contact with potential biohazards.
红细胞沉降率（ESR）仍然是监测感染、炎症性疾病和某些类型癌症最广泛使用的实验室检测方法。最近开发了几种检测方法，因此，ESR检测程序的安全性和可靠性得到了提高。本研究的目的是比较两种方法，即传统的手工魏氏法（国际血液学标准化委员会的参考方法）和一种新的半自动技术，即用于测定ESR的微量检测1法。
从200名住院和门诊患者中采集过夜空腹后的血样。使用用K3 EDTA抗凝的未稀释血样，其微量检测1值范围为2 - 82毫米/小时，与魏氏法进行比较。
比较微量检测1法和参考方法的线性回归分析得出样本具有令人满意的相关性和回归性（r = 0.910；P = 0.0001；y = 4.91 + 0.86x；Sy/x = 6.85）。Bland - Altman分析表明，微量检测1法和参考方法之间没有系统偏差的证据。
微量检测1系统易于使用，具有令人满意的操作实用性，维护需求最小，并减少了与潜在生物危害的接触。
