Anderson G P, King K D, Cao L K, Jacoby M, Ligler F S, Ezzell J
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, D.C., USA.
Clin Diagn Lab Immunol. 1998 Sep;5(5):609-12. doi: 10.1128/CDLI.5.5.609-612.1998.
The fiber-optic biosensor, originally developed to detect hazardous biological agents such as protein toxins or bacterial cells, has been utilized to quantify the concentration of serum antiplague antibodies. This biosensor has been used to detect and quantify the plague fraction 1 antigen in serum, plasma, and whole-blood samples, but its ability to quantify serum antibodies has not been demonstrated. By using a competitive assay, the concentration of serum antiplague antibodies was ascertained in the range of 2 to 15 microgram/ml. By making simple dilutions, concentrations for 11 serum samples whose antiplague antibody concentrations were unknown were determined and were found to be in good agreement with enzyme-linked immunosorbent assay results. The competitive assay method could be used to effectively determine the exposure to plague of animals or humans or could be applied to other diseases, such as hepatitis or AIDS, where the presence of antibodies is used to diagnose infection.
光纤生物传感器最初是为检测诸如蛋白质毒素或细菌细胞等有害生物制剂而开发的，现已用于定量血清抗鼠疫抗体的浓度。这种生物传感器已用于检测和定量血清、血浆及全血样本中的鼠疫F1抗原，但其定量血清抗体的能力尚未得到证实。通过竞争分析，确定血清抗鼠疫抗体的浓度范围为2至15微克/毫升。通过进行简单稀释，测定了11份抗鼠疫抗体浓度未知的血清样本的浓度，发现与酶联免疫吸附测定结果高度一致。竞争分析方法可有效确定动物或人类鼠疫暴露情况，也可应用于其他疾病，如肝炎或艾滋病，这些疾病可通过抗体的存在来诊断感染。
