Linfert D R, Wu A H, Tsongalis G J
Department of Pathology and Laboratory Medicine, Hartford Hospital, CT, USA.
J Forensic Sci. 1998 Sep;43(5):1041-5.
Human urine has not been adequately investigated as a potential source of DNA for forensic identity testing. The advent of polymerase chain reaction technology has made possible the analysis of previously undetectable levels of nucleic acids from human urine and other body fluids lacking nucleated cells. In this study, we evaluated the ability to genotype DNA extracted from adulterated urine specimens using the AmpliType PM + DQA1 PCR amplification and typing system. Fresh, first-void male urine specimens were contaminated with household bleach, E. coli, human serum albumin, glucose and saponin (a strong detergent). All of the adulterated samples were typed without difficulty. Frozen male urine specimens were split into equal volumes; one aliquot was adulterated with either E. coli or saponin, and the other was left free of contaminants. Seventy-one percent of all frozen urine specimens tested (adulterated and unadulterated) were successfully typed using this amplification and typing system. Our data, therefore, suggest that the AmpliType PM + DQA1 PCR amplification and typing system described is suitable for genotype analysis of adulterated fresh and frozen urine specimens.
人类尿液作为法医身份鉴定潜在的DNA来源尚未得到充分研究。聚合酶链反应技术的出现使得分析来自人类尿液和其他无核细胞体液中以前无法检测到的核酸水平成为可能。在本研究中，我们评估了使用AmpliType PM + DQA1 PCR扩增和分型系统对从掺假尿液标本中提取的DNA进行基因分型的能力。新鲜的首次晨尿男性标本被家用漂白剂、大肠杆菌、人血清白蛋白、葡萄糖和皂角苷（一种强力洗涤剂）污染。所有掺假样本都顺利进行了分型。将冷冻的男性尿液标本分成等份；一份用大肠杆菌或皂角苷掺假，另一份不添加污染物。使用该扩增和分型系统，所有测试的冷冻尿液标本（掺假和未掺假）中有71%成功进行了分型。因此，我们的数据表明，所描述的AmpliType PM + DQA1 PCR扩增和分型系统适用于掺假的新鲜和冷冻尿液标本的基因分型分析。
