巴西东南部HIV-1的GWG（脯氨酸-色氨酸）包膜变体的高频率。
High frequency of the GWG (Pro Trp) envelope variant of HIV-1 in Southeast Brazil.
作者信息
Covas D T, Bíscaro T A, Kashima S, Duarte G, Machado A A
机构信息
Regional Blood Center of Ribeirão Preto, and Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
出版信息
J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Sep 1;19(1):74-9. doi: 10.1097/00042560-199809010-00012.
The HIV-1 variant that contains the GWG amino acid sequence in the crown of the principal neutralizing determinant (PND) has been detected in a few patients in Japan, France, and Brazil by direct sequencing. We describe for the first time the use of restriction fragment length polymorphism (RFLP) and limited DNA sequencing of the C2-V3 region of the HIV-1 envelope (env) gene to determine the prevalence of the variant in 75 HIV-1-infected Brazilian patients. Overall prevalence of the GWG sequence as indicated by RFLP was 57% (43 of 75). The prevalence in females (72%) was higher than that in males (32%) and newborns (40%). Two GFG sequences and 1 GLG sequence were also detected. This finding is relevant for the planning of vaccines and for studies of the epidemiology of HIV-1 in Brazil.
在日本、法国和巴西的少数患者中，通过直接测序检测到了在主要中和决定簇（PND）顶端包含GWG氨基酸序列的HIV-1变体。我们首次描述了使用限制性片段长度多态性（RFLP）和对HIV-1包膜（env）基因C2-V3区域进行有限的DNA测序，以确定75名感染HIV-1的巴西患者中该变体的流行情况。RFLP显示的GWG序列总体流行率为57%（75例中的43例）。女性中的流行率（72%）高于男性（32%）和新生儿（40%）。还检测到两个GFG序列和一个GLG序列。这一发现对于巴西的疫苗规划和HIV-1流行病学研究具有重要意义。
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