Probst A J, Hertel C, Richter L, Wassill L, Ludwig W, Hammes W P
Institut für Lebensmitteltechnologie, Universität Hohenheim, Stuttgart, Germany.
Int J Syst Bacteriol. 1998 Jul;48 Pt 3:651-8. doi: 10.1099/00207713-48-3-651.
Based on the sequence data of 23S rRNA of Staphylococcus carnosus, Staphylococcus piscifermentans, Staphylococcus aureus and Staphylococcus epidermidis, species-specific probes were constructed. Their application revealed a heterogeneity within 18 strains previously identified as S. carnosus. Strains of this group were selected, and their 23S rRNA sequence was determined. It was revealed that the strains of S. carnosus can be placed in at least three sub-groups. This grouping was supported by physiological data and DNA-DNA similarity studies. Based on these results, were propose the new species Staphylococcus condimenti sp. nov. The type strain is S. condimenti F-2T (=DSM 11674T). The phylogenetic position of the new species within the radiation of other staphylococcal strains is reflected by a 16S nRNA-based tree. Furthermore, it is proposed to designate the new subspecies of Staphylococcus carnosus Schleifer and Fischer 1982, Staphylococcus carnosus subsp. utilis subsp. nov. The type strain of S. carnosus subsp. utilis is SK 11T (= DSM 11676T).
基于肉葡萄球菌、嗜鱼葡萄球菌、金黄色葡萄球菌和表皮葡萄球菌23S rRNA的序列数据，构建了种特异性探针。它们的应用揭示了18株先前鉴定为肉葡萄球菌的菌株存在异质性。选取了该组菌株并测定了它们的23S rRNA序列。结果表明，肉葡萄球菌菌株可至少分为三个亚组。这一分组得到了生理数据和DNA-DNA相似性研究的支持。基于这些结果，我们提出了新种调味品葡萄球菌（Staphylococcus condimenti sp. nov.）。模式菌株为调味品葡萄球菌F-2T（=DSM 11674T）。基于16S nRNA构建的树反映了新种在其他葡萄球菌菌株辐射范围内的系统发育位置。此外，建议将1982年施莱费尔和费舍尔命名的肉葡萄球菌的新亚种命名为肉葡萄球菌实用亚种（Staphylococcus carnosus subsp. utilis subsp. nov.）。肉葡萄球菌实用亚种的模式菌株为SK 11T（=DSM 11676T）。
