Marks S L, Williams D A
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616, USA.
Am J Vet Res. 1998 Sep;59(9):1113-5.
To establish values for gastrointestinal tract permeation by chromium 51-labeled ethylenediaminetetraacetate (51Cr-labeled EDTA) in healthy adult dogs, and to evaluate the time course for 51Cr-labeled EDTA absorption over a 24-hour period after its administration, in an effort to define a shorter, more practical collection method.
6 healthy adult mixed-breed dogs.
After an 18-hour nonfeeding period, each dog was given a solution containing 50 microCi of 51Cr-labeled EDTA in deionized water (10 ml/kg of body weight) by stomach tube. Complete urine collection was done at 2, 4, 6, and 24 hours after 51Cr-labeled EDTA administration. Five-milliliter samples of urine were counted for 15 minutes in a gamma counter, and radioactivity in urine was expressed as a percentage of the orally administered dose.
Median (range) 24-hour urinary recovery of 51Cr-labeled EDTA after 24 hours was 15.1 (12.7 to 20.3)%. Urine collected at 2, 4, and 6 hours contained 1.0 (0.2 to 3.5)%, 6.5 (2.2 to 8.7)%, and 10.0 (8.1 to 11.7)% of the administered 51Cr-labeled EDTA, respectively. Urine passed during the first 6 hours contained, on average, 67 (54 to 77)% of the total 24-hour urine recovery.
6-hour urinary recovery of 51Cr-EDTA provides a potential alternative to 24-hour recovery. This shorter collection period may more specifically reflect small intestinal permeability.
确定健康成年犬胃肠道对51铬标记乙二胺四乙酸（51Cr标记的EDTA）的渗透值，并评估51Cr标记的EDTA给药后24小时内的吸收时间进程，以确定一种更短、更实用的收集方法。
6只健康成年杂种犬。
在禁食18小时后，通过胃管给每只犬灌服含有50微居里51Cr标记的EDTA的去离子水溶液（10毫升/千克体重）。在给予51Cr标记的EDTA后2、4、6和24小时进行完整尿液收集。取5毫升尿液样本在γ计数器中计数15分钟，尿液中的放射性以口服剂量的百分比表示。
24小时后51Cr标记的EDTA的24小时尿回收率中位数（范围）为15.1%（12.7%至20.3%）。在2、4和6小时收集的尿液分别含有给药的51Cr标记的EDTA的1.0%（0.2%至3.5%）、6.5%（2.2%至8.7%）和10.0%（8.1%至11.7%）。前6小时排出的尿液平均占24小时总尿回收率的67%（54%至77%）。
51Cr-EDTA的6小时尿回收率可作为24小时回收率的潜在替代方法。这种较短的收集期可能更具体地反映小肠通透性。
