Frias Rafael, Sankari Satu, Westermarck Elias
Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
J Vet Intern Med. 2004 Mar-Apr;18(2):156-9. doi: 10.1892/0891-6640(2004)18<156:cabtae>2.0.co;2.
The 51Cr-EDTA test is a valuable clinical tool for screening intestinal diseases in dogs. The test is performed by calculating the percentage of recovery from urine of a PO-ingested dose of 51Cr-EDTA after 6 or 24 hours. Careful urine collection is a practical limitation of this test in dogs, and our goal was to develop a simpler test that measures 51Cr-EDTA in blood. A 51Cr-EDTA absorption test was simultaneously performed on urine and serum 43 times in healthy Beagle Dogs. Timed blood samples were withdrawn, and urine was collected during a 6-hour period. Percentages of the ingested dose were then calculated in urine and serum. The mean +/- standard deviation (range) percentage in urine after 6 hours was 14.07 +/- 8.72% (3.81-34.18%), whereas results in serum from samples taken at 2, 3, 4, 5, and 6 hours were 0.49 +/- 0.45% (0.02-2.13%), 0.75 +/- 0.52% (0.03-1.89%), 0.82 +/- 0.57% (0.13-2.21%), 0.70 +/- 0.53% (0.12-1.99%), and 0.47 +/- 0.44% (0.11-1.79%), respectively. The results for blood specimens showed good concordance with those for urine, especially for the samples taken at 4 hours (r = 0.89). Moreover, the correlation between urine and blood was better when the sum of the percentages of the recovered analyte from various blood samples was compared with urine. The correlation coefficient when summing 4 blood samples was excellent (r = 0.97) and remained excellent when summing only 2 blood samples taken at 3 and 5 hours (r = 0.95) or at 3 and 4 hours (r = 0.94). We conclude that a serum 51Cr-EDTA test determined by summing successive blood samples provides an easier means of estimating small intestinal permeability in dogs and gives results comparable to those of the 6-hour urine test.
51铬-乙二胺四乙酸（51Cr-EDTA）试验是筛查犬肠道疾病的一种有价值的临床工具。该试验通过计算口服51Cr-EDTA剂量后6或24小时尿液中的回收率来进行。在犬中，仔细收集尿液是该试验的一个实际限制因素，我们的目标是开发一种更简单的检测血液中51Cr-EDTA的试验。在43只健康的比格犬中同时进行了尿液和血清的51Cr-EDTA吸收试验。定时采集血样，并在6小时内收集尿液。然后计算尿液和血清中摄入剂量的百分比。6小时后尿液中的平均±标准差（范围）百分比为14.07±8.72%（3.81 - 34.18%），而在2、3、4、5和6小时采集的血清样本结果分别为0.49±0.45%（0.02 - 2.13%）、0.75±0.52%（0.03 - 1.89%）、0.82±0.57%（0.13 - 2.21%）、0.70±0.53%（0.12 - 1.99%）和0.47±0.44%（0.11 - 1.79%）。血液标本的结果与尿液结果显示出良好的一致性，尤其是在4小时采集的样本（r = 0.89）。此外，当将来自不同血样的回收分析物百分比之和与尿液进行比较时，尿液和血液之间的相关性更好。将4个血样相加时的相关系数极佳（r = 0.97），仅将在3和5小时采集的2个血样相加（r = 0.95）或在3和4小时采集的2个血样相加（r = 0.94）时，相关性仍然极佳。我们得出结论，通过将连续血样相加来测定血清51Cr-EDTA试验为评估犬小肠通透性提供了一种更简便的方法，其结果与6小时尿液试验的结果相当。
