McSharry J J, Lurain N S, Drusano G L, Landay A L, Notka M, O'Gorman M R, Weinberg A, Shapiro H M, Reichelderfer P S, Crumpacker C S
Albany Medical College, Albany, New York 12208, USA.
Antimicrob Agents Chemother. 1998 Sep;42(9):2326-31. doi: 10.1128/AAC.42.9.2326.
Rapid, quantitative, and objective determination of the susceptibilities of human cytomegalovirus (HCMV) clinical isolates to ganciclovir has been assessed by an assay that uses a fluorochrome-labeled monoclonal antibody to an HCMV immediate-early antigen and flow cytometry. Analysis of the ganciclovir susceptibilities of 25 phenotypically characterized clinical isolates by flow cytometry demonstrated that the 50% inhibitory concentrations (IC50s) of ganciclovir for 19 of the isolates were between 1.14 and 6.66 microM, with a mean of 4.32 microM (+/-1.93) (sensitive; IC50 less than 7 microM), the IC50s for 2 isolates were 8.48 and 9.79 microM (partially resistant), and the IC50s for 4 isolates were greater than 96 microM (resistant). Comparative analysis of the drug susceptibilities of these clinical isolates by the plaque reduction assay gave IC50s of less than 6 microM, with a mean of 2.88 microM (+/-1.40) for the 19 drug-sensitive isolates, IC50s of 6 to 8 microM for the partially resistant isolates, and IC50s of greater than 12 microM for the four resistant clinical isolates. Comparison of the IC50s for the drug-susceptible and partially resistant clinical isolates obtained by the flow cytometry assay with the IC50s obtained by the plaque reduction assay showed an acceptable correlation (r2 = 0.473; P = 0.001), suggesting that the flow cytometry assay could substitute for the more labor-intensive, subjective, and time-consuming plaque reduction assay.
一种利用针对人巨细胞病毒（HCMV）即刻早期抗原的荧光染料标记单克隆抗体和流式细胞术的检测方法，已被用于快速、定量且客观地测定HCMV临床分离株对更昔洛韦的敏感性。通过流式细胞术对25株经表型鉴定的临床分离株的更昔洛韦敏感性进行分析，结果显示，19株分离株的更昔洛韦50%抑制浓度（IC50）在1.14至6.66微摩尔之间，平均为4.32微摩尔（±1.93）（敏感；IC50小于7微摩尔），2株分离株的IC50为8.48和9.79微摩尔（部分耐药），4株分离株的IC50大于96微摩尔（耐药）。通过蚀斑减少试验对这些临床分离株的药物敏感性进行比较分析，19株药物敏感分离株的IC50小于6微摩尔，平均为2.88微摩尔（±1.40），部分耐药分离株的IC50为6至8微摩尔，4株耐药临床分离株的IC50大于12微摩尔。将流式细胞术检测获得的药物敏感和部分耐药临床分离株的IC50与蚀斑减少试验获得的IC50进行比较，结果显示具有可接受的相关性（r2 = 0.473；P = 0.001），这表明流式细胞术检测可替代更耗费人力、主观且耗时的蚀斑减少试验。
