Barawkar D A, Bruice T C
Department of Chemistry, University of California, Santa Barbara, CA 93106, USA.
Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11047-52. doi: 10.1073/pnas.95.19.11047.
The synthesis of mixed backbone oligodeoxynucleotides (18-mers) consisting of positively charged guanidinium linkages along with negatively charged phosphodiester linkages is carried out. The use of a base labile-protecting group for guanidinium linkage offers a synthetic strategy similar to standard oligonucleotide synthesis. The nuclease resistance of the oligodeoxyribonucleotides capped with guanidinium linkages at 5' and 3' ends are reported. The hybridization properties and sequence specificity of binding of these deoxynucleic guanidine/DNA chimeras with complementary DNA or RNA are described.
合成了由带正电荷的胍基连接和带负电荷的磷酸二酯连接组成的混合主链寡脱氧核苷酸(18聚体)。对胍基连接使用碱不稳定保护基团提供了一种类似于标准寡核苷酸合成的合成策略。报道了在5'和3'端用胍基连接封端的寡脱氧核糖核苷酸的核酸酶抗性。描述了这些脱氧核酸胍/DNA嵌合体与互补DNA或RNA结合的杂交特性和序列特异性。