采用气相色谱-质谱联用技术对血浆中5-氟尿嘧啶进行灵敏检测。
A sensitive assay of 5-fluorouracil in plasma by gas chromatography-mass spectrometry.
作者信息
Hillcoat B L, Kawai M, McCulloch P B, Rosenfeld J, Williams C K
出版信息
Br J Clin Pharmacol. 1976 Feb;3(1):135-43. doi: 10.1111/j.1365-2125.1976.tb00580.x.
Abstract
1 A gas chromatographic-mass spectrometric method was developed for determining 5-fluorouracil in plasma, using methylated thymine as an internal standard. 2 5-fluorouracil was extracted from plasma by a novel procedure which removed plasma components interferring with the sensitivity of the assay. The method included heating the plasma, washing with ether and extracting the drug under optimum conditions. 3 The sensitivity of the assay was 10 ng/ml plasma, sufficient to determine the low concentrations of 5-fluorouracil found in plasma during continuous infusion of the drug in patients receiving chemotherapy for cancer.
摘要
- 建立了一种气相色谱 - 质谱法，以甲基化胸腺嘧啶为内标物测定血浆中的5 - 氟尿嘧啶。2. 通过一种新方法从血浆中提取5 - 氟尿嘧啶，该方法可去除干扰测定灵敏度的血浆成分。该方法包括加热血浆、用乙醚洗涤并在最佳条件下提取药物。3. 该测定方法的灵敏度为10 ng/ml血浆，足以测定癌症化疗患者连续输注该药物期间血浆中低浓度的5 - 氟尿嘧啶。
相似文献
1
A sensitive assay of 5-fluorouracil in plasma by gas chromatography-mass spectrometry.
Br J Clin Pharmacol. 1976 Feb;3(1):135-43. doi: 10.1111/j.1365-2125.1976.tb00580.x.
2
Comparison of liquid- and gas-liquid chromatographic assays of 5-fluorouracil in plasma.
Clin Chem. 1979 Jan;25(1):83-6.
3
Determination of 5-fluorouracil in plasma by GC/MS using an internal standard. Applications to pharmacokinetics.
Bull Cancer. 1979;66(1):67-74.
4
A gas chromatographic assay for the determination of 5,6-dihydrofluorouracil and 5-fluorouracil in human plasma.
J Chromatogr. 1983 Dec 9;278(2):283-9. doi: 10.1016/s0378-4347(00)84787-8.
5
Quantitative analysis of 5-fluorouracil and 5,6-dihydrofluorouracil in plasma by gas chromatography mass spectrometry.
Biomed Mass Spectrom. 1982 Aug;9(8):336-9. doi: 10.1002/bms.1200090806.
6
Highly sensitive method for the determination of 5-fluorouracil in biological samples in the presence of 2'-deoxy-5-fluorouridine by gas chromatography-mass spectrometry.
J Chromatogr. 1991 Mar 8;564(1):137-45. doi: 10.1016/0378-4347(91)80076-o.
7
Gas chromatographic-mass spectrometric method for routine monitoring of 5-fluorouracil in plasma of patients receiving low-level protracted infusions.
J Chromatogr. 1992 Oct 23;581(2):195-201. doi: 10.1016/0378-4347(92)80272-r.
8
Comparison of gas chromatographic/mass spectrometric and microbiological assays for 5-fluorouracil in plasma.
Biomed Mass Spectrom. 1985 May;12(5):238-40. doi: 10.1002/bms.1200120510.
9
Comparative determinations of 5-fluorouracil in plasma using GC/MS and HPLC.
Arzneimittelforschung. 1981;31(12):2048-53.
10
Highly sensitive determination of 5-fluorouracil in human plasma by capillary gas chromatography and negative ion chemical ionization mass spectrometry.
J Chromatogr. 1985 Sep 13;343(1):59-66. doi: 10.1016/s0378-4347(00)84568-5.
引用本文的文献
1
Clinical response and plasma levels of 5-fluorouracil in patients with colonic cancer treated by drug infusion.
Br J Cancer. 1978 Dec;38(6):719-24. doi: 10.1038/bjc.1978.278.
2
Evidence for conversion of 5-fluorocytosine to 5-fluorouracil in humans: possible factor in 5-fluorocytosine clinical toxicity.
Antimicrob Agents Chemother. 1978 Dec;14(6):903-8. doi: 10.1128/AAC.14.6.903.
本文引用的文献
1
EXPERIMENTAL AND CLINICAL USE OF FLUORINATED PYRIMIDINES IN CANCER CHEMOTHERAPY.
Cancer Res. 1963 Sep;23:1226-43.
2
Countercurrent distribution.
Methods Biochem Anal. 1962;10:201-28. doi: 10.1002/9780470110270.ch7.
3
Gas chromatography of barbiturates, phenolic alkaloids, and xanthine bases: flash-heater methylation by means of trimethylanilinium hydroxide.
J Pharm Sci. 1969 Mar;58(3):370-1. doi: 10.1002/jps.2600580322.
4
GLC assay for 5-fluorouracil in biological fluids.
J Pharm Sci. 1973 Apr;62(4):572-5. doi: 10.1002/jps.2600620405.
5
Clinical pharmacology of oral and intravenous 5-fluorouracil (NSC-19893).
Cancer Chemother Rep. 1974 Sep-Oct;58(5 Pt 1):723-31.
6
Application of gas chromatography-chemical ionization mass fragmentography in the evaluation of bases and nucleoside analogues used in cancer chemotherapy.
J Chromatogr. 1974 Nov 6;99(0):519-27. doi: 10.1016/s0021-9673(00)90881-1.
7
Determination of 5-fluorouracil (NSC-19893) plasma levels in rats and man by isotope dilution-mass fragmentography.
Cancer Chemother Rep. 1975 Mar-Apr;59(2 Pt 1):279-86.
Zotero 插件