采用气相色谱-质谱联用技术对血浆中5-氟尿嘧啶进行灵敏检测。
A sensitive assay of 5-fluorouracil in plasma by gas chromatography-mass spectrometry.
作者信息
Hillcoat B L, Kawai M, McCulloch P B, Rosenfeld J, Williams C K
出版信息
Br J Clin Pharmacol. 1976 Feb;3(1):135-43. doi: 10.1111/j.1365-2125.1976.tb00580.x.
Abstract
1 A gas chromatographic-mass spectrometric method was developed for determining 5-fluorouracil in plasma, using methylated thymine as an internal standard. 2 5-fluorouracil was extracted from plasma by a novel procedure which removed plasma components interferring with the sensitivity of the assay. The method included heating the plasma, washing with ether and extracting the drug under optimum conditions. 3 The sensitivity of the assay was 10 ng/ml plasma, sufficient to determine the low concentrations of 5-fluorouracil found in plasma during continuous infusion of the drug in patients receiving chemotherapy for cancer.
摘要
- 建立了一种气相色谱 - 质谱法，以甲基化胸腺嘧啶为内标物测定血浆中的5 - 氟尿嘧啶。2. 通过一种新方法从血浆中提取5 - 氟尿嘧啶，该方法可去除干扰测定灵敏度的血浆成分。该方法包括加热血浆、用乙醚洗涤并在最佳条件下提取药物。3. 该测定方法的灵敏度为10 ng/ml血浆，足以测定癌症化疗患者连续输注该药物期间血浆中低浓度的5 - 氟尿嘧啶。
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本文引用的文献
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EXPERIMENTAL AND CLINICAL USE OF FLUORINATED PYRIMIDINES IN CANCER CHEMOTHERAPY.氟代嘧啶在癌症化疗中的实验与临床应用
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Countercurrent distribution.逆流分布
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Gas chromatography of barbiturates, phenolic alkaloids, and xanthine bases: flash-heater methylation by means of trimethylanilinium hydroxide.
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GLC assay for 5-fluorouracil in biological fluids.生物流体中5-氟尿嘧啶的葡萄糖醛酸苷测定法。
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Application of gas chromatography-chemical ionization mass fragmentography in the evaluation of bases and nucleoside analogues used in cancer chemotherapy.气相色谱-化学电离质谱碎片分析法在癌症化疗中使用的碱基和核苷类似物评估中的应用
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Determination of 5-fluorouracil (NSC-19893) plasma levels in rats and man by isotope dilution-mass fragmentography.通过同位素稀释-质谱碎片分析法测定大鼠和人体中5-氟尿嘧啶（NSC-19893）的血浆水平。
Cancer Chemother Rep. 1975 Mar-Apr;59(2 Pt 1):279-86.
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