乙琥胺、苯巴比妥、扑米酮和苯妥英的同步定量气相色谱分析。
Simultaneous quantitative gas-chromatographic analysis of ethosuximide, phenobarbitone, primidone and diphenylhydantoin.
作者信息
Latham A N, Varlow G
出版信息
Br J Clin Pharmacol. 1976 Feb;3(1):145-50. doi: 10.1111/j.1365-2125.1976.tb00581.x.
1 Therapeutic serum concentrations of ethosuximide, phenobarbitone, primidone, and dipheylhydantoin were assayed from 1 ml of human serum. The extraction procedure was common to all four drugs and three internal standards. 2 Subsequent isothermal gas chromatographic analysis of serum extracts produced well resolved peaks for the underivatized quantitation of ethosuximide and phenobarbitone. Primidone and diphenylhydantoin were determined as methylated derivatives. 3 Mean coefficients of variation for the assay of each drug were less 7% on a newly packed and conditioned column and less than 10% after the technique had been in continuous use for 3 months. 4 The advantage of quantitation relative to peak area ratios rather than peak height ratios was minimal for the determination of ethosuximide, primidone and diphenylhydantoin but appeared significant for the assay of phenobarbitone.
- 从1毫升人血清中测定了乙琥胺、苯巴比妥、扑米酮和苯妥英的治疗血清浓度。提取程序适用于所有四种药物和三种内标。2. 随后对血清提取物进行等温气相色谱分析，得到了用于乙琥胺和苯巴比妥未衍生化定量的分辨率良好的峰。扑米酮和苯妥英作为甲基化衍生物进行测定。3. 在新填充和调节的色谱柱上，每种药物测定的平均变异系数小于7%，该技术连续使用3个月后小于10%。4. 对于乙琥胺、扑米酮和苯妥英的测定，相对于峰高比，采用峰面积比进行定量的优势极小，但对于苯巴比妥的测定似乎很显著。
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