Liu H, Huang T, Kissinger C B, Kissinger P T
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
J Chromatogr B Biomed Sci Appl. 1998 Aug 25;713(2):289-95. doi: 10.1016/s0378-4347(98)00167-4.
A liquid chromatographic method has been developed for the determination of 3-nitro-L-tyrosine. Different detection methods, including UV, oxidative and redox electrochemistry, and postcolumn photolysis followed by electrochemical detection, have been optimized and compared in terms of analysis time, detection limit and dynamic range. It was demonstrated that liquid chromatography with postcolumn photolysis followed by electrochemical detection is the most effective method, with an analysis time of 5 min, detection limit of 0.01 pmol, and a linear dynamic range from 2 nM to 100 microM.
已开发出一种用于测定3-硝基-L-酪氨酸的液相色谱方法。已对包括紫外、氧化和氧化还原电化学以及柱后光解后电化学检测在内的不同检测方法在分析时间、检测限和动态范围方面进行了优化和比较。结果表明，柱后光解后电化学检测的液相色谱法是最有效的方法，分析时间为5分钟，检测限为0.01皮摩尔，线性动态范围为2纳摩尔至100微摩尔。
