用于液相色谱法测定3-硝基-L-酪氨酸的柱后光解和电化学检测的优化

Optimization of post-column photolysis and electrochemical detection for the liquid chromatographic determination of 3-nitro-L-tyrosine.

作者信息

Liu H, Duda C T, Huang T, Aruda W O, Kissinger P T

机构信息

Department of Chemistry, Peking University, Beijing, China.

出版信息

J Chromatogr A. 1998 Aug 28;818(1):69-75. doi: 10.1016/s0021-9673(98)00487-7.

DOI:10.1016/s0021-9673(98)00487-7

PMID:9770305

Abstract

A new post-column photolysis technology has been developed based on the use of a low pressure, low temperature UV lamp and TiO2 coated knitted reaction coil. As a test case the developed technique was used for the determination of 3-nitro-L-tyrosine by liquid chromatography with electrochemical detection. Different photolysis lamps and reactor tubing lengths were evaluated in terms of their effect on the separation efficiency and/or photolysis efficiency. A detection limit of 0.5 nM (10 fmol) for 3-nitro-L-tyrosine was achieved under optimized conditions, with a linear correlation coefficient of R2 = 0.9898 over a concentration range of 2-100 microM. Pre-injection photolysis of 3-nitro-L-tyrosine indicated that dihydroxyphenylalanine is the main photolysis product. In general, use of the photoreactor prior to liquid chromatography is an excellent method for exploring photodegradation products of an analyte in conjunction with the full range of available liquid chromatography detectors.

摘要

基于低压、低温紫外灯和二氧化钛涂层针织反应线圈，开发了一种新的柱后光解技术。作为一个测试案例，所开发的技术用于通过液相色谱 - 电化学检测法测定3 - 硝基 - L - 酪氨酸。评估了不同的光解灯和反应器管长对分离效率和/或光解效率的影响。在优化条件下，3 - 硝基 - L - 酪氨酸的检测限为0.5 nM（10 fmol），在2 - 100 microM的浓度范围内线性相关系数R2 = 0.9898。3 - 硝基 - L - 酪氨酸的进样前光解表明二羟基苯丙氨酸是主要的光解产物。一般来说，在液相色谱之前使用光反应器是结合所有可用的液相色谱检测器探索分析物光降解产物的极佳方法。