Tsuboi H, Kouda K, Takeuchi H, Takigawa M, Masamoto Y, Takeuchi M, Ochi H
Department of Public Health, Hamamatsu University School of Medicine, Shizuoka-ken, Japan.
Br J Dermatol. 1998 Jun;138(6):1033-5. doi: 10.1046/j.1365-2133.1998.02273.x.
8-hydroxydeoxyguanosine (8-OHdG) is one of the products which are excreted in urine as a result of oxidative damage to DNA. We investigated the feasibility of using 8-OHdG in urine as an index for oxidative damage to DNA in atopic dermatitis (AD). Seventeen patients with long-standing AD and 17 healthy volunteers were enrolled in this study. The severity of AD was evaluated by SCORAD index. Eosinophils, total IgE and lactate dehydrogenase-5 in peripheral blood were measured as clinical parameters for AD. A newly developed enzyme-linked immunosorbent assay method was used to measure urine 8-OHdG. The AD patients showed significantly higher levels (P < 0.0001) of 8-OHdG in their urine than corresponding controls. Urine 8-OHdG levels showed as strong a positive correlation as other haematological parameters did using the SCORAD index. Thus, we conclude that the urine 8-OHdG levels can also serve as a biochemical index of tissue damage and can act as a useful tool in the clinical evaluation of AD.
8-羟基脱氧鸟苷（8-OHdG）是DNA氧化损伤后经尿液排出的产物之一。我们研究了将尿液中的8-OHdG作为特应性皮炎（AD）患者DNA氧化损伤指标的可行性。本研究纳入了17例慢性AD患者和17名健康志愿者。采用SCORAD指数评估AD的严重程度。检测外周血嗜酸性粒细胞、总IgE和乳酸脱氢酶-5作为AD的临床参数。采用新开发的酶联免疫吸附测定法检测尿液中的8-OHdG。AD患者尿液中8-OHdG水平显著高于相应对照组（P < 0.0001）。尿液8-OHdG水平与使用SCORAD指数的其他血液学参数呈同样强的正相关。因此，我们得出结论，尿液8-OHdG水平也可作为组织损伤的生化指标，并可作为AD临床评估的有用工具。
