Montine T J, Markesbery W R, Morrow J D, Roberts L J
Department of Pathology, and Center for Molecular Neurosciences, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Ann Neurol. 1998 Sep;44(3):410-3. doi: 10.1002/ana.410440322.
Postmortem studies have associated Alzheimer's disease (AD) with regionally increased oxidative damage to brain. Lacking, however, is a specific marker of oxidative damage to brain that may be measured during life. We tested the hypothesis that cerebrospinal fluid (CSF) concentrations of F2-isoprostanes (F2-IsoPs), stable products of arachidonate peroxidation, are increased in CSF of AD patients. CSF from lateral ventricles (VF) was analyzed from 11 AD patients and 11 control subjects who participated in a rapid autopsy program. VF F2-IsoP concentrations were significantly elevated in AD patients compared with control subjects (72 +/- 7 vs 46 +/- 4 pg/ml) and were significantly linearly correlated with brain weight (-0.3 pg/ml/g, r2 = 0.32). These results suggest that quantification of CSF F2-IsoP concentrations may provide a useful biomarker of central nervous system oxidative damage in AD.
尸检研究已将阿尔茨海默病（AD）与大脑局部氧化损伤增加联系起来。然而，目前尚缺乏一种可在生前测量的大脑氧化损伤特异性标志物。我们检验了这样一个假设：即AD患者脑脊液（CSF）中F2 - 异前列腺素（F2 - IsoPs）的浓度会升高，F2 - IsoPs是花生四烯酸过氧化的稳定产物。对参与快速尸检项目的11名AD患者和11名对照受试者的侧脑室（VF）脑脊液进行了分析。与对照受试者相比，AD患者的VF F2 - IsoP浓度显著升高（分别为72±7与46±4 pg/ml），并且与脑重量显著线性相关（-0.3 pg/ml/g，r2 = 0.32）。这些结果表明，脑脊液F2 - IsoP浓度的定量测定可能为AD中枢神经系统氧化损伤提供一种有用的生物标志物。
