Hofbauer R, Moser D, Hammerschmidt V, Kapiotis S, Frass M
Department of General Anesthesiology and Intensive Care, Clinical Institute of Medical and Chemical Laboratory Diagnostics, School of Medicine, The University of Vienna, Austria.
Crit Care Med. 1998 Sep;26(9):1545-9. doi: 10.1097/00003246-199809000-00022.
To study the role of neutrophils in host defense, using human endothelial cells in migration studies in the presence of ketamine (0.3, 3, and 30 microg/mL).
Prospective, controlled study.
University research laboratories.
Seven independent experiments from different donors were done, investigating the influence of ketamine (0.3, 3, and 30 microg/mL) to the migration of human leukocytes through human endothelial cell monolayers.
Human endothelial cell monolayers and/or human leukocytes were preincubated with clinically relevant (3 microg/mL), higher (30 microg/mL), and lower (0.3 microg/mL) concentrations of ketamine. The amount of leukocyte migration after 3 hrs was measured in a fluorometer.
Human endothelial cells isolated from umbilical veins were cultured on microporous membranes (polyethylene-terephthalat membranes) until they formed an endothelial cell monolayer. Leukocytes were separated by standard procedures. The migration of leukocytes through monolayers of endothelial cells under the clinically relevant concentration of ketamine was reduced to 59+/-9.8% (SD) (p< .05) when leukocytes but not the endothelial cell monolayers were preincubated with ketamine. Leukocyte migration was reduced to 92+/-7.3% (p > .05) when only monolayers of endothelial cells were treated with ketamine, and to 52+/-8.8% (p< .05) when both leukocytes and monolayers of endothelial cells were treated with ketamine. The higher and lower concentrations showed a dose-dependent effect.
We investigated the cellular interaction between both cell systems, leukocytes and endothelial cells, simultaneously in the presence of ketamine. Ketamine is able to reduce significantly the migration of leukocytes through endothelial cell monolayers. The use of different dosages revealed a dose-dependent effect. The current model allowed treatment of one cell type, either leukocyte or endothelial cell. Ketamine inhibits the function of leukocytes more than the function of endothelial cells.
在氯胺酮（0.3、3和30微克/毫升）存在的情况下，利用人内皮细胞进行迁移研究，以探讨中性粒细胞在宿主防御中的作用。
前瞻性对照研究。
大学研究实验室。
进行了来自不同供体的7项独立实验，研究氯胺酮（0.3、3和30微克/毫升）对人白细胞通过人内皮细胞单层迁移的影响。
将人内皮细胞单层和/或人白细胞与临床相关浓度（3微克/毫升）、较高浓度（30微克/毫升）和较低浓度（0.3微克/毫升）的氯胺酮进行预孵育。3小时后，在荧光计中测量白细胞迁移量。
从脐静脉分离的人内皮细胞在微孔膜（聚对苯二甲酸乙二酯膜）上培养，直至形成内皮细胞单层。白细胞通过标准程序分离。当白细胞而非内皮细胞单层与氯胺酮预孵育时，在临床相关浓度的氯胺酮作用下，白细胞通过内皮细胞单层的迁移减少至59±9.8%（标准差）（p<0.05）。当仅内皮细胞单层用氯胺酮处理时，白细胞迁移减少至92±7.3%（p>0.05），而当白细胞和内皮细胞单层均用氯胺酮处理时，白细胞迁移减少至52±8.8%（p<0.05）。较高和较低浓度呈现剂量依赖性效应。
我们在氯胺酮存在的情况下，同时研究了白细胞和内皮细胞这两种细胞系统之间的细胞相互作用。氯胺酮能够显著减少白细胞通过内皮细胞单层的迁移。使用不同剂量显示出剂量依赖性效应。当前模型允许对一种细胞类型，即白细胞或内皮细胞进行处理。氯胺酮对白细胞功能的抑制作用大于对内皮细胞功能的抑制作用。
