Hofbauer R, Moser D, Salfinger H, Frass M, Kapiotis S
Department of Anesthesiology and Intensive Care Medicine, University of Vienna, Austria.
Intensive Care Med. 1998 Sep;24(9):973-6. doi: 10.1007/s001340050698.
The interactions between blood and vascular wall cells are essential for the understanding of pathophysiologic processes, e.g. inflammation. The influence of the anesthetic drug thiopental on leukocyte function is well documented. Recently, an inhibitory effect of thiopental on leukocyte chemotaxis in a Boyden chamber assay (i.e. endothelial cells were not included) was demonstrated. In vivo, leukocytes have to interact with endothelial cell monolayers to invade the tissue. The influence of thiopental on a monolayer of endothelial cells has not yet been investigated. The aim of the current study was to investigate the influence of thiopental on the migration of leukocytes through endothelial cell monolayers (ECM).
Human umbilical vein endothelial cells (HUVEC) were isolated and cultured on microporous membrane filters to achieve a monolayer. Isolated polymorphonuclear leukocytes (PMNL) as well as ECM were preincubated with different concentrations of thiopental. The rate of leukocyte migration against the chemotactic protein formyl-methyl-leucyl-phenylalanine was measured (n = 7). Thiopental was able to reduce the amount of leukocyte migration through ECM significantly.
In conclusion, we could show that thiopental is able to reduce the migration of PMNL through ECM significantly.
血液与血管壁细胞之间的相互作用对于理解病理生理过程（如炎症）至关重要。麻醉药物硫喷妥钠对白细胞功能的影响已有充分记录。最近，在Boyden小室试验（即未包含内皮细胞）中证实了硫喷妥钠对白细胞趋化性有抑制作用。在体内，白细胞必须与内皮细胞单层相互作用才能侵入组织。硫喷妥钠对内皮细胞单层的影响尚未得到研究。本研究的目的是探讨硫喷妥钠对白细胞通过内皮细胞单层（ECM）迁移的影响。
分离人脐静脉内皮细胞（HUVEC）并在微孔膜滤器上培养以形成单层。将分离的多形核白细胞（PMNL）以及ECM与不同浓度的硫喷妥钠进行预孵育。测量白细胞针对趋化蛋白甲酰-甲基-亮氨酰-苯丙氨酸的迁移速率（n = 7）。硫喷妥钠能够显著降低白细胞通过ECM的迁移量。
总之，我们能够证明硫喷妥钠能够显著降低PMNL通过ECM的迁移。
