Huber R, Eder W, Heldwein S, Wanner G, Huber H, Rachel R, Stetter KO
Lehrstuhl fur Mikrobiologie and Archaeenzentrum, Universitat Regensburg, D-93053 Regensburg, Germany.
Appl Environ Microbiol. 1998 Oct;64(10):3576-83. doi: 10.1128/AEM.64.10.3576-3583.1998.
A novel hyperthermophilic bacterium was isolated from pink filamentous streamers (pink filaments) occurring in the upper outflow channel (temperature, 82 to 88 degreesC) of Octopus Spring in Yellowstone National Park, Wyo. The gram-negative cells grew at low salinity at temperatures up to 89 degreesC in the neutral to alkaline pH range. Depending on the culture conditions, the organisms occurred as single motile rods, as aggregates, or as long filaments that formed streamer-like cell masses. The novel isolate grew chemolithoautotrophically with hydrogen, thiosulfate, and elemental sulfur as electron donors and oxygen as the electron acceptor. Alternatively, under aerobic conditions, formate and formamide served as sole energy and carbon sources. The novel isolate had a 16S rRNA sequence closely related to the 16S rRNA sequence obtained from uncultivated pink filaments. It represents a new genus in the order Aquificales, the type species of which we name Thermocrinis ruber (type strain, OC 1/4 [= DSM 12173]).
从怀俄明州黄石国家公园章鱼泉上游流出通道（温度为82至88摄氏度）中出现的粉红色丝状飘带（粉红色细丝）中分离出一种新型嗜热细菌。革兰氏阴性细胞在低盐度下，于中性至碱性pH范围内，温度高达89摄氏度时生长。根据培养条件，这些生物体以单个游动杆状、聚集体或形成丝状飘带样细胞团的长丝形式存在。这种新型分离株以氢气、硫代硫酸盐和元素硫作为电子供体，以氧气作为电子受体进行化能自养生长。另外，在有氧条件下，甲酸盐和甲酰胺作为唯一的能量和碳源。这种新型分离株的16S rRNA序列与从未培养的粉红色细丝中获得的16S rRNA序列密切相关。它代表了 Aquificales 目中的一个新属，我们将其模式种命名为 Thermocrinis ruber（模式菌株，OC 1/4 [= DSM 12173]）。
