de la Fuente R, García A, Ruiz-Santa-Quiteria J A, Luzón M, Cid D, García S, Orden J A, Gómez-Bautista M
Departamento Patología Animal 1, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Prev Vet Med. 1998 Aug 7;36(2):145-52. doi: 10.1016/s0167-5877(98)00077-4.
Faecal samples from 218 diarrheic dairy calves in 65 dairy herds, selected by convenience, were screened for the presence of rotavirus, coronavirus, Cryptosporidium spp., F5+ Escherichia coli and Salmonella spp. Animals surveyed were from 1 to 30 days old. Cryptosporidium and rotavirus were the most commonly detected agents (52.3% and 42.7% of the samples positive, respectively). F5+ E. coli was detected in the faeces of 11.9% of the calves and bovine coronavirus was detected in the faeces of 7.3% of the calves. Salmonella spp. was only found in the faeces of two calves (0.9%). Mixed infections with two or more agents occurred in 28% of the calves. Concurrent infection of rotavirus and Cryptosporidium was found in 21.6% of the calves. Two tests were used for the detection of rotavirus (a commercial ELISA and PAGE), F5+ E. coli (ELISA and bacterial culture) and Cryptosporidium (ELISA and microscopy). The validity of the commercial ELISA for the detection of rotavirus, F5+ E. coli and Cryptosporidium in faeces from diarrheic calves was evaluated using PAGE, bacterial culture and microscopy as gold standard, respectively. The ELISA showed a very low sensitivity (28.6%) for the detection of F5+ E. coli compared to bacterial culture.
通过便利抽样，从65个奶牛场的218头腹泻奶牛犊中采集粪便样本，检测其中是否存在轮状病毒、冠状病毒、隐孢子虫属、F5 +大肠杆菌和沙门氏菌属。所调查的动物年龄在1至30日龄之间。隐孢子虫和轮状病毒是最常检测到的病原体（分别占样本阳性的52.3%和42.7%）。在11.9%的犊牛粪便中检测到F5 +大肠杆菌，在7.3%的犊牛粪便中检测到牛冠状病毒。沙门氏菌属仅在两头犊牛（0.9%）的粪便中发现。28%的犊牛发生了两种或更多病原体的混合感染。在21.6%的犊牛中发现轮状病毒和隐孢子虫的同时感染。使用两种检测方法检测轮状病毒（一种商业ELISA和PAGE）、F5 +大肠杆菌（ELISA和细菌培养）和隐孢子虫（ELISA和显微镜检查）。分别以PAGE、细菌培养和显微镜检查作为金标准，评估商业ELISA检测腹泻犊牛粪便中轮状病毒、F5 +大肠杆菌和隐孢子虫的有效性。与细菌培养相比，ELISA检测F5 +大肠杆菌的灵敏度非常低（28.6%）。
