Nagatani T, Okazawa H, Kambara T, Satoh K, Nishiyama T, Tokura H, Yamada R, Nakajima H
Department of Dermatology, Yokohama City University School of Medicine, Yokohama, Japan.
Melanoma Res. 1998 Aug;8(4):295-9. doi: 10.1097/00008390-199808000-00001.
Malignant melanoma is one of the fulminant skin cancers. The 5-year survival of patients with stage III (N0, N1) malignant melanoma treated with multi-agent chemoimmunotherapy, including natural interferon-beta (nIFNbeta), was found in our department to be better than that of patients treated with other forms of therapy. In order to study the effects of nIFNbeta on melanoma, the growth inhibition effect of nIFNbeta was assessed in vitro using the melanoma cell lines, MM8.1, MM28, MM33.1, Bowes and A375-2. The growth of these cell lines was inhibited by nIFNbeta. Incorporation of [3H]thymidine and [3H]uridine was also inhibited by nIFNbeta in a dose-dependent manner. Apoptosis was demonstrated using the TUNEL method in melanoma cell lines cocultured with nIFNbeta. Results showed that nIFNbeta had direct killer activity on melanoma cell lines.
恶性黑色素瘤是一种侵袭性很强的皮肤癌。在我们科室发现，接受包括天然β干扰素（nIFNβ）在内的多药化疗免疫疗法治疗的III期（N0、N1）恶性黑色素瘤患者的5年生存率高于接受其他治疗形式的患者。为了研究nIFNβ对黑色素瘤的影响，使用黑色素瘤细胞系MM8.1、MM28、MM33.1、Bowes和A375 - 2在体外评估了nIFNβ的生长抑制作用。这些细胞系的生长受到nIFNβ的抑制。nIFNβ还以剂量依赖的方式抑制了[3H]胸腺嘧啶核苷和[3H]尿苷的掺入。使用TUNEL法在与nIFNβ共培养的黑色素瘤细胞系中证实了细胞凋亡。结果表明，nIFNβ对黑色素瘤细胞系具有直接杀伤活性。
