Verger J M, Grayon M, Tibor A, Wansard V, Letesson J J, Cloeckaert A
Laboratoire de Pathologie infectieuse et Immunologie, Institut National de la Recherche Agronomique, Nouzilly, France.
Res Microbiol. 1998 Jul-Aug;149(7):509-17. doi: 10.1016/s0923-2508(98)80005-3.
The possibility of differentiating Brucella species and biovars by Southern blot hybridization of agarose gel-electrophoresed HindIII-digested genomic DNA with membrane protein- or cytoplasmic protein-specific gene probes was investigated on 92 reference and field strains representative of all known species and biovars. Based on the RFLP pattern observed, three gene probes, i.e. br25, 39ugpa and omp16 coding for membrane or cytoplasmic proteins differentiated B. melitensis, B. ovis and B. suis biovar 2 strains from each other and from the other Brucella species and biovars. Thus, the use of these specific gene probes could contribute, in addition to previously identified species- or biovar-specific markers, to the molecular identification and typing of Brucella isolates.
利用膜蛋白或胞质蛋白特异性基因探针，通过对琼脂糖凝胶电泳分离的经HindIII消化的基因组DNA进行Southern印迹杂交，对代表所有已知种和生物变种的92株参考菌株和现场菌株进行布鲁氏菌物种和生物变种鉴别可能性的研究。基于观察到的限制性片段长度多态性（RFLP）图谱，三种编码膜蛋白或胞质蛋白的基因探针，即br25、39ugpa和omp16，可将羊种布鲁氏菌、绵羊种布鲁氏菌和猪种布鲁氏菌生物变种2菌株相互区分开来，并与其他布鲁氏菌物种和生物变种区分开来。因此，除了先前鉴定的物种或生物变种特异性标记外，使用这些特异性基因探针有助于布鲁氏菌分离株的分子鉴定和分型。
