[用于测定抗麻疹病毒总抗体的间接超微量酶联免疫吸附测定法的标准化]
[Standardization of an indirect ultramicro ELISA for the determination of total antibodies against the measles virus].
作者信息
Soler Nodarse M, Domínguez D R, Vázquez Ramudo S, Laferté Serrano J, Guzmán Tirado M G
机构信息
Instituto de Medicina Tropical Pedro Kouri.
出版信息
Rev Cubana Med Trop. 1992;44(3):192-6.
An indirect ultramicro ELISA system (UME) for the determination of antibodies to measles virus by the cuban technology of Ultramicro Analytical Systems (SUMA) was developed. A number of 448 serum samples from the National System of Seroepidemiological Surveillance of Measles were simultaneously processed by the Ultramicro ELISA system and by the haemagglutination inhibition technique. Results were compared in both assays. Afterwards; 120 serum pairs obtained from the same source were studies and processed in a similar way. Results evidenced the usefulness of such immunoenzymatic system for the serological diagnosis and the positive validation of replacing conventional techniques by this novel technology.
开发了一种采用古巴超微分析系统技术（SUMA）测定麻疹病毒抗体的间接超微酶联免疫吸附测定系统（UME）。来自国家麻疹血清流行病学监测系统的448份血清样本同时采用超微酶联免疫吸附测定系统和血凝抑制技术进行处理。对两种检测方法的结果进行了比较。之后，对从同一来源获得的120对血清进行了研究，并以类似方式进行处理。结果证明了这种免疫酶系统在血清学诊断中的实用性，以及用这种新技术替代传统技术的阳性验证。
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