Gravet A, Colin D A, Keller D, Girardot R, Monteil H, Prévost G
UPRES EA-1318, Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur-Hôpitaux Universitaires de Strasbourg, France.
FEBS Lett. 1998 Oct 2;436(2):202-8. doi: 10.1016/s0014-5793(98)01130-2.
A new member of the staphylococcal bi-component leucotoxins family, LukE (32 kDa) and LukD (34.3 kDa) has been characterized from Staphylococcus aureus strain Newman. LukE was 58-68% identical with the class S proteins, whereas LukD was 71-77% identical with the class F proteins of the family. A partial immunoreactivity with the various affinity-purified antibodies specific for the other proteins was observed. Immunoprecipitation assay and gene probing confirmed a 30% frequency among human clinical isolates, differing from the distribution of the other known leucotoxins (P<0.005). LukE+LukD was as effective as the Panton-Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus.
已从金黄色葡萄球菌纽曼菌株中鉴定出葡萄球菌双组分白细胞毒素家族的一个新成员，即LukE（32 kDa）和LukD（34.3 kDa）。LukE与S类蛋白的同源性为58% - 68%，而LukD与该家族F类蛋白的同源性为71% - 77%。观察到它与针对其他蛋白的各种亲和纯化抗体存在部分免疫反应性。免疫沉淀试验和基因探测证实，在人类临床分离株中的出现频率为30%，这与其他已知白细胞毒素的分布不同（P<0.005）。当注射到兔皮内时，LukE+LukD诱导皮肤坏死的效果与杀白细胞素相当，但与金黄色葡萄球菌表达的其他白细胞毒素相比，它没有溶血活性且白细胞毒性较弱。
