Hucker H B, Stauffer S C
J Pharm Sci. 1976 Aug;65(8):1253-5. doi: 10.1002/jps.2600650832.
A GLC determination of cyclobenzaprine in human plasma and urine is described. After extraction from alkalinized samples with heptane-isopentyl alcohol (97:3), the drug and internal standard were back-extracted into 0.1 N HCl and then reextracted into ether. Use of a lower homolog of the drug as an internal standard was effective in reducing variability. Drug concentrations as low as 25 ng/ml could be assayed with high precision. Plasma levels in humans given 40 mg po or iv ranged from 5 to 51 ng/ml; little unchanged cyclobenzaprine was present in the urine. The N-desmethyl analog of the drug was detected as a metabolite in urine.
本文描述了一种测定人血浆和尿液中环苯扎林的气相色谱法。用庚烷 - 异戊醇（97:3）从碱化样品中萃取后，将药物和内标反萃取到0.1N盐酸中，然后再萃取到乙醚中。使用该药物的低级同系物作为内标可有效降低变异性。低至25 ng/ml的药物浓度也能高精度测定。口服或静脉注射40 mg的人体血浆水平在5至51 ng/ml之间；尿液中几乎没有未变化的环苯扎林。该药物的N - 去甲基类似物在尿液中被检测为代谢物。
