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丝状真菌体外药敏试验的接种物制备

Inoculum preparation for in-vitro susceptibility testing of filamentous fungi.

作者信息

Guarro J, Pujol I, Aguilar C, Llop C, Fernández-Ballart J

机构信息

Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.

出版信息

J Antimicrob Chemother. 1998 Sep;42(3):385-7. doi: 10.1093/jac/42.3.385.

DOI:10.1093/jac/42.3.385
PMID:9786479
Abstract

The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10(5) and 1-5 x 10(4) conidia/mL, adjusted with a haemocytometer, were determined. A wide variation of T values was observed, especially with the first inoculum. A good agreement was obtained between the counted and the viable propagules. The haemocytometric method is considered a useful technique for standardizing inocula.

摘要

测定了125株具有临床重要性的丝状真菌两种悬浮液的百分透射率(T)以及每毫升菌落形成单位(cfu/mL)的等效值,这两种悬浮液经血细胞计数器调整后分别含有1 - 5×10⁵和1 - 5×10⁴个分生孢子/mL。观察到T值有很大差异,尤其是第一种接种物。计数的繁殖体与活繁殖体之间取得了良好的一致性。血细胞计数法被认为是一种用于标准化接种物的有用技术。

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