Inoculum preparation for in-vitro susceptibility testing of filamentous fungi.

The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10(5) and 1-5 x 10(4) conidia/mL, adjusted with a haemocytometer, were determined. A wide variation of T values was observed, especially with the first inoculum. A good agreement was obtained between the counted and the viable propagules. The haemocytometric method is considered a useful technique for standardizing inocula.