Aberkane A, Cuenca-Estrella M, Gomez-Lopez A, Petrikkou E, Mellado E, Monzón A, Rodriguez-Tudela J L
Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain.
J Antimicrob Chemother. 2002 Nov;50(5):719-22. doi: 10.1093/jac/dkf187.
Two different methods of inoculum preparation for susceptibility testing were analysed. The first method was adjustment of inoculum size by haemocytometer counting. The second method was spectrophotometric adjustment at 530 nm. The reliability of both methods was assessed by colony counting. The overall agreement between colony counting and haemocytometer counts was 93.6%, and the intraclass coefficient correlation was 0.71 (P < 0.05). Pearson's correlation index between colony counts and optical density values was -0.059 (P > 0.05). Optical densities ranged between 0.01 and 1.2, showing less reproducibility than expected by the NCCLS M 38-P standard. Haemocytometer counting is a more reliable method of inoculum preparation for antifungal susceptibility testing.
分析了两种用于药敏试验的接种物制备方法。第一种方法是通过血细胞计数器计数来调整接种物大小。第二种方法是在530nm处进行分光光度法调整。通过菌落计数评估了这两种方法的可靠性。菌落计数与血细胞计数器计数之间的总体一致性为93.6%，组内相关系数为0.71（P<0.05）。菌落计数与光密度值之间的Pearson相关指数为-0.059（P>0.05）。光密度范围在0.01至1.2之间，显示出的可重复性低于NCCLS M 38-P标准预期。血细胞计数器计数是用于抗真菌药敏试验的更可靠的接种物制备方法。
