丝状真菌体外药敏试验接种体制备的分光光度法
Spectrophotometric method of inoculum preparation for the in vitro susceptibility testing of filamentous fungi.
作者信息
Espinel-Ingroff A, Kerkering T M
机构信息
Division of Infectious Diseases, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0049.
出版信息
J Clin Microbiol. 1991 Feb;29(2):393-4. doi: 10.1128/jcm.29.2.393-394.1991.
Abstract
Homogeneous inoculum suspensions of 29 isolates of clinically important filamentous fungi were adjusted with a spectrophotometer (530 nm) to obtain standardized preparations containing 1 x 10(6) to 5 x 10(6) CFU/ml. Colony counts (CFU per milliliter) of 1 x 10(6) to 5 x 10(6) were achieved on three different days for isolates of Aspergillus spp., Pseudallescheria boydii, and Sporothrix schenckii (80% +/- 2% transmission), and colony counts of 7 x 10(5) to 2.9 x 10(6) (70% +/- 2% transmission) were achieved for Mucor spp. and Rhizopus spp.
摘要
使用分光光度计（530纳米）对29株具有临床重要性的丝状真菌的均匀接种物悬液进行调整，以获得含有1×10⁶至5×10⁶CFU/毫升的标准化制剂。对于烟曲霉属、波氏假阿利什霉和申克孢子丝菌的分离株，在三天内实现了1×10⁶至5×10⁶的菌落计数（每毫升CFU）（透光率80%±2%），而对于毛霉属和根霉属，实现了7×10⁵至2.9×10⁶（透光率70%±2%）的菌落计数。
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