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本文引用的文献

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Variables influencing susceptibility testing of Cryptococcus neoformans to 5-fluorocytosine.影响新型隐球菌对5-氟胞嘧啶药敏试验的变量。
Antimicrob Agents Chemother. 1973 Oct;4(4):392-5. doi: 10.1128/AAC.4.4.392.
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Comparison of relative susceptibilities of Candida species to three antifungal agents as determined by unstandardized methods.用非标准化方法测定念珠菌属对三种抗真菌药物的相对敏感性比较。
Antimicrob Agents Chemother. 1987 Sep;31(9):1343-7. doi: 10.1128/AAC.31.9.1343.
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Multicenter evaluation of four methods of yeast inoculum preparation.四种酵母接种体制备方法的多中心评估。
J Clin Microbiol. 1988 Aug;26(8):1437-41. doi: 10.1128/jcm.26.8.1437-1441.1988.
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New fluorescence assay for the quantitation of fungi.用于定量真菌的新型荧光检测法。
J Clin Microbiol. 1989 Sep;27(9):2003-7. doi: 10.1128/jcm.27.9.2003-2007.1989.
5
Standardization of yeast inocula with an electronic impedance counter.使用电子阻抗计数器对酵母接种物进行标准化。
J Clin Microbiol. 1989 Oct;27(10):2397-9. doi: 10.1128/jcm.27.10.2397-2399.1989.
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Collaborative investigation of variables in susceptibility testing of yeasts.酵母药敏试验中变量的协作研究。
Antimicrob Agents Chemother. 1990 Sep;34(9):1648-54. doi: 10.1128/AAC.34.9.1648.
7
Turbidimetric studies of growth inhibition of yeasts with three drugs: inquiry into inoculum-dependent susceptibility testing, time of onset of drug effect, and implications for current and newer methods.三种药物对酵母生长抑制的比浊法研究：接种量依赖性药敏试验探究、药物作用起效时间及对现有和新方法的启示
Antimicrob Agents Chemother. 1978 Feb;13(2):249-54. doi: 10.1128/AAC.13.2.249.

丝状真菌体外药敏试验接种体制备的分光光度法

Spectrophotometric method of inoculum preparation for the in vitro susceptibility testing of filamentous fungi.

作者信息

Espinel-Ingroff A, Kerkering T M

机构信息

Division of Infectious Diseases, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0049.

出版信息

J Clin Microbiol. 1991 Feb;29(2):393-4. doi: 10.1128/jcm.29.2.393-394.1991.

DOI:10.1128/jcm.29.2.393-394.1991
PMID:2007647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269774/
Abstract

Homogeneous inoculum suspensions of 29 isolates of clinically important filamentous fungi were adjusted with a spectrophotometer (530 nm) to obtain standardized preparations containing 1 x 10(6) to 5 x 10(6) CFU/ml. Colony counts (CFU per milliliter) of 1 x 10(6) to 5 x 10(6) were achieved on three different days for isolates of Aspergillus spp., Pseudallescheria boydii, and Sporothrix schenckii (80% +/- 2% transmission), and colony counts of 7 x 10(5) to 2.9 x 10(6) (70% +/- 2% transmission) were achieved for Mucor spp. and Rhizopus spp.

摘要

使用分光光度计（530纳米）对29株具有临床重要性的丝状真菌的均匀接种物悬液进行调整，以获得含有1×10⁶至5×10⁶CFU/毫升的标准化制剂。对于烟曲霉属、波氏假阿利什霉和申克孢子丝菌的分离株，在三天内实现了1×10⁶至5×10⁶的菌落计数（每毫升CFU）（透光率80%±2%），而对于毛霉属和根霉属，实现了7×10⁵至2.9×10⁶（透光率70%±2%）的菌落计数。