Dawson D A, Carman J, Collins J, Hill S, Lappin M R
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft. Collins 80523, USA.
J Vet Diagn Invest. 1998 Oct;10(4):315-9. doi: 10.1177/104063879801000401.
Feline herpesvirus 1 (FHV) is an important cause of feline ocular and respiratory disease, but the role the virus plays in central nervous system disease of cats has not been explored. The study described here was performed to validate an indirect enzyme-linked immunosorbent assay (ELISA) to detect FHV-specific IgG antibodies for use in feline epidemiologic, ocular, and central nervous system disease studies. The indirect IgG ELISA was applied to serum, aqueous humor, and cerebrospinal fluid from cats. Serum FHV IgG ELISA results were compared with those of serum neutralization in client-owned cats and laboratory-housed cats following vaccination. Of the 100 client-owned cats tested by ELISA, 97 had detectable FHV IgG; 95 had titers >32. The FHV IgG ELISA was more sensitive than serum neutralization and could be used with aqueous humor and cerebrospinal fluid. Cats with inflammatory central nervous system or ocular diseases had significant leakage of serum proteins into aqueous humor and cerebrospinal fluid, necessitating use of cutoff values derived from serum when these fluids were assessed.
猫疱疹病毒1型（FHV）是猫眼部和呼吸道疾病的重要病因，但该病毒在猫中枢神经系统疾病中所起的作用尚未得到研究。本文所述的研究旨在验证一种间接酶联免疫吸附测定（ELISA），以检测用于猫流行病学、眼部和中枢神经系统疾病研究的FHV特异性IgG抗体。将间接IgG ELISA应用于猫的血清、房水和脑脊液。将血清FHV IgG ELISA结果与接种疫苗后的客户拥有猫和实验室饲养猫的血清中和结果进行比较。在通过ELISA检测的100只客户拥有猫中，97只检测到FHV IgG；95只滴度>32。FHV IgG ELISA比血清中和更敏感，可用于房水和脑脊液检测。患有炎症性中枢神经系统或眼部疾病的猫，血清蛋白会大量漏入房水和脑脊液，因此在评估这些液体时需要使用源自血清的临界值。
