Guyard-Dangremont V, Desrumaux C, Gambert P, Lallemant C, Lagrost L
Heart Institute, Ottawa Civic Hospital, Ontario, Canada.
Comp Biochem Physiol B Biochem Mol Biol. 1998 Jul;120(3):517-25. doi: 10.1016/s0305-0491(98)10038-x.
Cholesteryl ester and phospholipid transfer activities were determined in plasmas from 14 vertebrates, and lipid transfer values were analyzed in the light of the known atherogenesis susceptibility of studied species. Whereas cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP) activities among vertebrate species were only measured in lipoprotein-deficient fractions in previous studies, both endogenous lipoprotein-dependent and endogenous lipoprotein-independent assays were used in the present work. In agreement with previous studies, a few species (chicken, man, rabbit and trout) displayed substantial CETP activity, whereas CETP activity was not detectable in other species (cow, dog, horse, mouse, pig, and rat). Additional species that were not studied before, i.e. cat, goat, and sheep, were shown to be deficient in plasma cholesteryl ester transfer activity, while duck was shown to constitute a new member of the high activity group. Unlike CETP activity, PLTP activity was detected in plasmas from all studied species, most of them being assayed here for the first time (cat, chicken, cow, duck, goat, horse, sheep, and trout). While dog, trout, mouse, and pig displayed the highest phospholipid transfer activity levels, the remarkable preservation of facilitated phospholipid transfers in plasma from all vertebrates might indicate an essential role of PLTP in vivo. Interestingly, animals with well-documented atherogenesis susceptibility (chicken, pig, rabbit, and man) displayed significantly higher mean CETP activity, but lower mean PLTP activity than known 'resistant' animals (cat, dog, mouse, and rat). In conclusion, the present study revealed marked differences in plasma lipid transfer activities between vertebrate species, and interspecies comparisons indicated that both CETP and PLTP may constitute two determinants of the atherogenicity of the plasma lipoprotein profile.
测定了14种脊椎动物血浆中的胆固醇酯和磷脂转移活性，并根据所研究物种已知的动脉粥样硬化易感性分析了脂质转移值。在以往的研究中，脊椎动物物种中的胆固醇酯转移蛋白（CETP）和磷脂转移蛋白（PLTP）活性仅在脂蛋白缺陷组分中进行了测量，而本研究使用了内源性脂蛋白依赖性和内源性脂蛋白非依赖性两种检测方法。与以往的研究一致，一些物种（鸡、人、兔和鳟鱼）表现出显著的CETP活性，而在其他物种（牛、狗、马、小鼠、猪和大鼠）中未检测到CETP活性。之前未研究过的其他物种，即猫、山羊和绵羊，被证明血浆胆固醇酯转移活性缺乏，而鸭被证明是高活性组的新成员。与CETP活性不同，所有研究物种的血浆中均检测到PLTP活性，其中大多数物种（猫、鸡、牛、鸭、山羊、马、绵羊和鳟鱼）在此首次进行检测。虽然狗、鳟鱼、小鼠和猪表现出最高的磷脂转移活性水平，但所有脊椎动物血浆中促进磷脂转移的显著保留可能表明PLTP在体内具有重要作用。有趣的是，具有充分记录的动脉粥样硬化易感性的动物（鸡、猪、兔和人）的平均CETP活性显著更高，但平均PLTP活性低于已知的“抗性”动物（猫、狗、小鼠和大鼠）。总之，本研究揭示了脊椎动物物种之间血浆脂质转移活性的显著差异，种间比较表明，CETP和PLTP可能都是血浆脂蛋白谱致动脉粥样硬化性的两个决定因素。
