Speijer H, Groener J E, van Ramshorst E, van Tol A
Department of Biochemistry, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The Netherlands.
Atherosclerosis. 1991 Oct;90(2-3):159-68. doi: 10.1016/0021-9150(91)90110-o.
Activities of cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP) were measured in plasma of four vertebrate species: man, rabbit, pig, and rat. The activities were measured in the absence and presence of antibodies raised against purified human CETP. PLTP activities were present in all four species with highest values in pig (11.7 +/- 1.2 U/ml) and human plasma (9.2 +/- 1.6 U/ml). Considerable lower activities were found in rabbit (3.5 +/- 0.6 U/ml) and rat plasma (1.6 +/- 0.7 U/ml). These activities were not affected significantly by antibody against human CETP. CETP activities could be measured in human (0.23 +/- 0.05 U/ml) and in rabbit plasma (0.19 +/- 0.03 U/ml). CETP activity in human plasma was inhibited over 97% by antibody against human CETP. Plasma was chromatographed on a Superose 6 gel filtration column. Average HDL particle sizes in the four species differed notably and decreased in the order: rat HDL greater than rabbit HDL greater than human HDL greater than pig HDL. A separation of the two lipid transfer activities was evident after gel filtration chromatography. The peak of the PLTP activity coeluted with a fraction of HDL particles with the size of human HDL2 (particle weights 300-375 kDa). CETP activity in human and rabbit plasma coeluted largely with relatively small HDL particles (particle weights 140-180 kDa). These results show that CETP and PLTP activities are located in different macromolecular complexes.
在人、兔、猪和大鼠这四种脊椎动物的血浆中测量了胆固醇酯转运蛋白（CETP）和磷脂转运蛋白（PLTP）的活性。在不存在和存在针对纯化的人CETP产生的抗体的情况下测量这些活性。所有四种物种中均存在PLTP活性，其中猪血浆（11.7±1.2 U/ml）和人血浆（9.2±1.6 U/ml）中的活性最高。在兔血浆（3.5±0.6 U/ml）和大鼠血浆（1.6±0.7 U/ml）中发现的活性明显较低。这些活性不受抗人CETP抗体的显著影响。可以在人血浆（0.23±0.05 U/ml）和兔血浆（0.19±0.03 U/ml）中测量CETP活性。抗人CETP抗体可抑制人血浆中97%以上的CETP活性。血浆在Superose 6凝胶过滤柱上进行色谱分析。这四种物种中的平均高密度脂蛋白（HDL）颗粒大小显著不同，并且按以下顺序降低：大鼠HDL＞兔HDL＞人HDL＞猪HDL。凝胶过滤色谱后，两种脂质转运活性明显分离。PLTP活性峰与一部分大小与人HDL2（颗粒重量300 - 375 kDa）相同的HDL颗粒共洗脱。人血浆和兔血浆中的CETP活性主要与相对较小的HDL颗粒（颗粒重量140 - 180 kDa）共洗脱。这些结果表明，CETP和PLTP活性位于不同的大分子复合物中。
