Jauh G Y, Fischer A M, Grimes H D, Ryan C A, Rogers J C
Institute of Biological Chemistry, Box 6340, Washington State University, Pullman, WA 99164, USA.
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12995-9. doi: 10.1073/pnas.95.22.12995.
Plant cell vacuoles may have either storage or degradative functions. Vegetative storage proteins (VSPs) are synthesized in response to wounding and to developmental switches that affect carbon and nitrogen sinks. Here we show that VSPs are stored in a unique type of vacuole that is derived from degradative central vacuoles coincident with insertion of a new tonoplast intrinsic protein (TIP), delta-TIP, into their membranes. This finding demonstrates a tight coupling between the presence of delta-TIP and acquisition of a specialized storage function and indicates that TIP isoforms may determine vacuole identity.
植物细胞液泡可能具有储存或降解功能。营养储存蛋白（VSPs）是在受伤以及影响碳和氮库的发育转变时合成的。我们在此表明，VSPs 储存在一种独特的液泡中，这种液泡源自降解性的中央液泡，同时有新的液泡膜内在蛋白（TIP），即δ-TIP，插入其膜中。这一发现证明了δ-TIP 的存在与获得专门储存功能之间的紧密联系，并表明 TIP 同工型可能决定液泡的特性。
