Arima K, Uéda K, Sunohara N, Hirai S, Izumiyama Y, Tonozuka-Uehara H, Kawai M
Department of Ultrastructure and Histochemistry, Tokyo Institute of Psychiatry, 2-1-8 Kamikitazawa, Setagaya-ku, Tokyo 156-8585, Japan.
Brain Res. 1998 Oct 12;808(1):93-100. doi: 10.1016/s0006-8993(98)00734-3.
We examined brains from Parkinson's disease and from dementia with Lewy bodies (LBs) by using antibodies to NACP/alpha-synuclein. Immunohistochemically, all of the antibodies against the amino-terminal region, NAC domain, and carboxyl-terminal region of NACP labeled not only LBs, pale bodies (PBs), and dystrophic neurites, but also fine thread-like structures in the neuronal perikarya (perikaryal threads) in the hypothalamus and brainstem nuclei. On electron microscopy, immunoreactive products were found to label the 9 to 12 nm-thick filamentous component (LB-filaments) of LBs, PBs, and perikaryal threads. The NACP-immunoreactive perikaryal threads, consisting of small bundles of LB-filaments and randomly oriented LB-filaments, presumably represent an initial stage of LB- or PB-formation. The present study indicates that the entire molecule of NACP is involved in the neuronal filament-aggregating processes of LB disorders.
我们使用针对NACP/α-突触核蛋白的抗体，对帕金森病和路易体痴呆（LBs）患者的大脑进行了检查。免疫组织化学研究显示，所有针对NACP氨基末端区域、NAC结构域和羧基末端区域的抗体，不仅标记了路易小体（LBs）、苍白小体（PBs）和营养不良性神经突，还标记了下丘脑和脑干核中神经元胞体（胞体细丝）内的细丝状结构。电子显微镜检查发现，免疫反应产物标记了LBs、PBs和胞体细丝中9至12纳米厚的丝状成分（LB细丝）。由小束LB细丝和随机排列的LB细丝组成的NACP免疫反应性胞体细丝，可能代表了LB或PB形成的初始阶段。本研究表明，NACP的整个分子参与了LB疾病的神经元细丝聚集过程。
