Fasano M, Theise N D, Nalesnik M, Goswami S, Garcia de Davila M T, Finegold M J, Greco M A
Department of Pathology, New York University Medical Center, New York 10016, USA.
Mod Pathol. 1998 Oct;11(10):934-8.
The distinction of hepatoblastoma, especially the embryonal type, from other small, round-cell tumors of childhood can sometimes be difficult. Polyclonal anticarcinoembryonic antigen (pCEA) and Hepatocyte Paraffin 1 (Hep Par 1) are immunohistochemical markers that are useful in the diagnosis of hepatocellular carcinomas. We immunohistochemically studied pCEA, monoclonal CEA (mCEA), and Hep Par 1 on 12 hepatoblastomas (3 fetal type, 2 embryonal type, and 7 mixed epithelial type). In addition, we studied the expression of Hep Par 1 on 27 other selected childhood tumors, including 1 hepatocellular carcinoma, 5 germ-cell tumors, 4 peripheral neuroectodermal tumors/Ewing's sarcomas, 3 rhabdomyosarcomas, 5 neuroblastomas, 2 rhabdoid tumors, 3 lymphomas, and 4 Wilms' tumors. All of the hepatoblastomas expressed Hep Par 1 with a characteristic granular intracytoplasmic pattern that was generally less intense in embryonal-type than in fetal-type hepatoblastomas, perhaps reflecting the degree of hepatocyte differentiation. All of the fetal-type hepatoblastomas expressed pCEA with both an intracytoplasmic and bile canalicular pattern. Embryonal type hepatoblastomas were more likely to be pCEA negative or to show focal or no canalicular pattern of expression, again possibly reflecting the degree of hepatocyte differentiation. All of the hepatoblastomas were mCEA negative. All of the nonhepatoblastomas were Hep Par 1 negative, except for the one hepatocellular carcinoma in this study, which was Hep Par 1 positive. We conclude that Hep Par 1 and pCEA are useful markers for hepatoblastomas, as they have been shown to be in hepatocellular carcinomas.
肝母细胞瘤，尤其是胚胎型，与儿童期其他小圆形细胞肿瘤的鉴别有时会很困难。多克隆癌胚抗原（pCEA）和肝细胞石蜡1（Hep Par 1）是有助于诊断肝细胞癌的免疫组化标志物。我们对12例肝母细胞瘤（3例胎儿型、2例胚胎型和7例混合上皮型）进行了pCEA、单克隆CEA（mCEA）和Hep Par 1的免疫组化研究。此外，我们还研究了Hep Par 1在27例其他选定的儿童肿瘤中的表达，包括1例肝细胞癌、5例生殖细胞肿瘤、4例外周神经外胚层肿瘤/尤因肉瘤、3例横纹肌肉瘤、5例神经母细胞瘤、2例横纹肌样瘤、3例淋巴瘤和4例肾母细胞瘤。所有肝母细胞瘤均表达Hep Par 1，呈特征性的颗粒状胞浆模式，胚胎型肝母细胞瘤的表达强度通常低于胎儿型，这可能反映了肝细胞的分化程度。所有胎儿型肝母细胞瘤均表达pCEA，呈胞浆内和胆小管模式。胚胎型肝母细胞瘤更可能pCEA阴性或呈局灶性或无胆小管模式表达，同样可能反映了肝细胞的分化程度。所有肝母细胞瘤均为mCEA阴性。除本研究中的1例肝细胞癌Hep Par 1阳性外，所有非肝母细胞瘤均为Hep Par 1阴性。我们得出结论，Hep Par 1和pCEA是肝母细胞瘤的有用标志物，正如它们在肝细胞癌中所显示的那样。
