Dennie D, Gladu I, Lepine F, Villemur R, Bisaillon J, Beaudet R
Centre de Recherche en Microbiologie Appliquee, Institut Armand-Frappier, Universite du Quebec, Ville de Laval, Quebec, Canada H7N 4Z3.
Appl Environ Microbiol. 1998 Nov;64(11):4603-6. doi: 10.1128/AEM.64.11.4603-4606.1998.
Desulfitobacterium frappieri PCP-1 was induced for ortho- and para-dechlorinating activities by different chlorophenols. Dehalogenation rates ranging from 25 to 1,158 nmol/min/mg of cell protein were observed according to the chlorophenol tested and the position of the chlorine removed. D. frappieri shows a broad substrate specificity; in addition to tetrachloroethylene and pentachloropyridine, strain PCP-1 can dehalogenate at ortho, meta, and para positions a large variety of aromatic molecules with substituted hydroxyl or amino groups. Reactions of O demethylation and reduction of nitro to amino substituents on aromatic molecules were also observed.
弗氏脱硫杆菌PCP-1可被不同的氯酚诱导产生邻位和对位脱氯活性。根据所测试的氯酚以及去除氯的位置,观察到脱卤速率在25至1158 nmol/分钟/毫克细胞蛋白之间。弗氏脱硫杆菌表现出广泛的底物特异性;除了四氯乙烯和五氯吡啶外,PCP-1菌株还可以在邻位、间位和对位使多种带有取代羟基或氨基的芳香分子脱卤。还观察到了芳香分子上的O-去甲基化反应以及硝基还原为氨基取代基的反应。