Murayama S, Yagyu S, Higo K, Ye C, Mizuno T, Oyabu A, Ito M, Morita H, Maeda K, Serikawa T, Matsuyama M
Department of Pathology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake 470-1192, Japan.
Mamm Genome. 1998 Nov;9(11):886-8. doi: 10.1007/s003359900888.
The BUF/Mna (BUF) strain is a high-proteinuria line of rats, and virtually all rats develop overt proteinuria by the age of 20 weeks. Genetic analysis revealed that proteinuria susceptibility was determined principally by two autosomal recessive genes. These findings prompted us to perform genetic mapping of the genes. (BUF/Mna x WKY/NCrj) F1 x BUF/Mna backcross rats were raised and maintained for 40-60 weeks to detect proteinuria. DNAs were extracted from ears of these rats and were examined by linkage study with polymerase chain reaction (PCR) with 132 microsatellite markers. Fifty-three out of 167 rats developed proteinuria. DNAs of 51 out of these 53 rats showed homozygous BUF/BUF genotype in the D13Mgh4 and D13N1 markers located on Chromosome (Chr) 13. The D13Rat1, D13Mgh2, D13Rat13, D13Mgh3, Syt2, Ren, D13Rat25, D13Mit2, D13Mgh5, and D13N2 markers located on the chromosome also showed statistically significant linkage to the development of proteinuria, whereas the other 110 markers showed no linkage. Here we report that a proteinuria-susceptible gene, Pur1, resides on a region flanked by the loci D13Mgh3 and D13Mgh4 on Chr 13.
BUF/Mna（BUF）品系是大鼠的高蛋白尿品系，几乎所有大鼠在20周龄时都会出现明显的蛋白尿。遗传分析表明，蛋白尿易感性主要由两个常染色体隐性基因决定。这些发现促使我们对这些基因进行遗传定位。将（BUF/Mna×WKY/NCrj）F1×BUF/Mna回交大鼠饲养并维持40至60周以检测蛋白尿。从这些大鼠的耳部提取DNA，并使用132个微卫星标记通过聚合酶链反应（PCR）进行连锁研究。167只大鼠中有53只出现蛋白尿。这53只大鼠中的51只在位于13号染色体（Chr）上的D13Mgh4和D13N1标记中显示纯合的BUF/BUF基因型。位于该染色体上的D13Rat1、D13Mgh2、D13Rat13、D13Mgh3、Syt2、Ren、D13Rat25、D13Mit2、D13Mgh5和D13N2标记也显示出与蛋白尿的发生有统计学意义的连锁关系，而其他110个标记则没有连锁关系。在此我们报告，一个蛋白尿易感基因Pur1位于13号染色体上由D13Mgh3和D13Mgh4位点侧翼的区域。
