Watanabe M, Takano T, Nakamura K
Department of Public Health and Environmental Science, School of Medicine, Tokyo Medical and Dental University, Japan.
J Toxicol Environ Health A. 1998 Oct 23;55(4):297-305. doi: 10.1080/009841098158458.
The effect of 2 mM ethanol, a concentration indicative of daily alcohol consumption, was investigated on trichloroethylene (TRI) metabolism in perfused Wistar rat liver. The study consisted of two parts: The first part studied TRI administration with or without ethanol. In the second study chloral hydrate (CH), an intermediate in TRI metabolism, was administered in the absence or presence of ethanol to phenobarbital (PB) treated or non-PB-treated rats. The concentrations of the metabolites, total trichloroethanol (TCE), and trichloroacetic acid (TCA) were measured by gas chromatography and intracellular reduced pyridine nucleotides by surface fluorometry. In the first study, ethanol infusion significantly increased the TCE/TCA ratio, TCE production rate, and percentage of reduced pyridine nucleotides, and decreased TCA production rate without an associated change in the sum of TCE and TCA formation rates. In the second study, ethanol infusion in the absence or presence of PB produced similar significant increases in the TCE/TCA ratio, TCE production rate, and percentage of reduced pyridine nucleotides, accompanied by a decrease in TCA formation. The observed shift in TRI metabolism in the presence of ethanol, from oxidation to TCA to reduction to TCE, suggests that alcohol exerts alterations in hepatic intracellular oxidation-reduction (redox) states.
研究了2 mM乙醇（代表日常酒精摄入量的浓度）对灌注的Wistar大鼠肝脏中三氯乙烯（TRI）代谢的影响。该研究包括两个部分：第一部分研究了给予TRI时有无乙醇的情况。在第二项研究中，在给予或不给予乙醇的情况下，向经苯巴比妥（PB）处理或未经PB处理的大鼠给予三氯乙醛（CH，TRI代谢的中间产物）。通过气相色谱法测量代谢产物、总三氯乙醇（TCE）和三氯乙酸（TCA）的浓度，通过表面荧光法测量细胞内还原型吡啶核苷酸的浓度。在第一项研究中，输注乙醇显著增加了TCE/TCA比值、TCE生成率和还原型吡啶核苷酸的百分比，并降低了TCA生成率，而TCE和TCA生成率之和没有相关变化。在第二项研究中，无论有无PB，输注乙醇都会使TCE/TCA比值、TCE生成率和还原型吡啶核苷酸的百分比显著增加，同时TCA生成减少。在乙醇存在的情况下观察到的TRI代谢从氧化生成TCA转变为还原生成TCE，表明酒精会改变肝脏细胞内的氧化还原状态。
