Egenvall A, Bjöersdorff A, Lilliehöök I, Olsson Engvall E, Karlstam E, Artursson K, Hedhammar A, Gunnarsson A
Department of Small Animal Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Vet Rec. 1998 Oct 10;143(15):412-7. doi: 10.1136/vr.143.15.412.
Seven beagles were inoculated experimentally with a Swedish canine Ehrlichia species isolate to study its pathogenicity. With respect to the 16S rRNA gene sequence, the isolate was identical to the human granulocytic ehrlichiosis (HGE) agent and closely related to both Ehrlichia equi and E phagocytophila. After an incubation period of four to 11 days, the most prominent clinical signs were high fever for two to five days and depression. All the dogs developed profound thrombocytopenia, moderate leukopenia and a strong serological antibody response. Ehrlichial inclusions were detected in blood neutrophils from four to 14 days after inoculation for four to eight days. Ehrlichial DNA could be detected by polymerase chain reaction during the parasitaemic stage and a few days before and after microscopic inclusions were visible. Postmortem, the dogs showed reactive splenic hyperplasia and non-specific mononuclear reactive hepatitis.
七只比格犬被实验性接种瑞典犬埃立克体物种分离株以研究其致病性。就16S rRNA基因序列而言，该分离株与人类粒细胞埃立克体病（HGE）病原体相同，并且与马埃立克体和嗜吞噬细胞埃立克体密切相关。在四至十一天的潜伏期后，最突出的临床症状是持续两至五天的高烧和抑郁。所有犬只均出现严重血小板减少、中度白细胞减少和强烈的血清学抗体反应。接种后四至十四天，在血液中性粒细胞中检测到埃立克体包涵体，持续四至八天。在寄生虫血症阶段以及显微镜下可见包涵体之前和之后的几天，可以通过聚合酶链反应检测到埃立克体DNA。死后检查发现，这些犬只表现出反应性脾增生和非特异性单核细胞反应性肝炎。
