大肠杆菌cmlA基因编码多药外排泵Cmr/MdfA，并负责异丙基-β-D-硫代半乳糖苷的排除和壮观霉素敏感性。
The Escherichia coli cmlA gene encodes the multidrug efflux pump Cmr/MdfA and is responsible for isopropyl-beta-D-thiogalactopyranoside exclusion and spectinomycin sensitivity.
作者信息
Bohn C, Bouloc P
机构信息
Laboratoire des Réseaux de Régulations, Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS/URA2225, F-91405 Orsay Cedex, France.
出版信息
J Bacteriol. 1998 Nov;180(22):6072-5. doi: 10.1128/JB.180.22.6072-6075.1998.
Abstract
Expression of cloned genes from isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/Cmr/MdfA efflux pump is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype. cmlA1 contains an IS30 insertion upstream of cmr/mdfA, which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity.
摘要
当大肠杆菌CmlA/Cmr/MdfA外排泵过表达时，来自异丙基-β-D-硫代半乳糖苷（IPTG）调控启动子的克隆基因的表达会降低，这可能是由于IPTG被排除在细胞质之外。先前报道的cmlA1突变赋予了类似的表型。cmlA1在cmr/mdfA上游含有一个IS30插入序列，该序列产生一个推定的启动子。CmlA的过量产生也会导致对壮观霉素过敏。
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