Wong A C
Department of Food Microbiology and Toxicology, University of Wisconsin, Madison, USA.
J Dairy Sci. 1998 Oct;81(10):2765-70. doi: 10.3168/jds.s0022-0302(98)75834-5.
Biofilms are a constant concern in food processing environments. Our overall research focus has been to understand the interaction of factors affecting bacterial attachment and biofilm formation with the ultimate goal of devising strategies to control this problem. This paper briefly describes three areas of biofilm research in which we have been involved. Listeria monocytogenes, a foodborne pathogen, survived for prolonged periods on stainless steel and buna-n rubber, materials commonly used in food-processing equipment. Survival was affected by temperature, relative humidity, attachment surface, and soil. Some components in the rubber inhibited growth of the organism on buna-n, which also affected the efficacy of sanitizers on biofilm inactivation. In cheese manufacture, biofilms of Lactobacillus curvatus could lead to a defect caused by the formation of calcium lactate crystals in Cheddar cheese. This hardy organism persisted in low numbers on equipment surfaces and was difficult to eradicate. We investigated the relative contributions and interactions of mechanical, thermal, and chemical processes in an air-injected clean-in-place method for milking systems. Overall, it is important to study the interactions between bacteria and the surfaces in a specific food processing environment to provide more effective measures for prevention of biofilm formation and for its removal.
生物膜一直是食品加工环境中令人担忧的问题。我们的总体研究重点是了解影响细菌附着和生物膜形成的因素之间的相互作用，最终目标是制定控制这一问题的策略。本文简要介绍了我们所参与的生物膜研究的三个领域。食源性病原体单核细胞增生李斯特菌能在不锈钢和丁腈橡胶（食品加工设备常用材料）上长期存活。其存活受温度、相对湿度、附着表面和污垢的影响。橡胶中的某些成分会抑制该菌在丁腈橡胶上的生长，这也会影响消毒剂对生物膜灭活的效果。在奶酪生产中，弯曲乳杆菌生物膜可能导致切达干酪中形成乳酸钙晶体，从而产生缺陷。这种顽强的微生物会在设备表面少量存活，且难以根除。我们研究了机械、热和化学过程在挤奶系统的空气注入就地清洗方法中的相对作用和相互作用。总体而言，研究特定食品加工环境中细菌与表面之间的相互作用，对于提供预防生物膜形成及其去除的更有效措施非常重要。
